Development and characterization of novel, polymorphic microsatellite markers for oat crown rust, Puccinia coronata

Hattie R. Dambroski, Martin L Carson

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

We report the development of 37 novel and polymorphic microsatellite markers for oat crown rust, Puccinia coronata f.sp. avenae. The allelic diversity ranged from two to 16 alleles per locus. Observed heterozygosity ranged from 0.000 to 0.971, and expected heterozygosity from 0.057 to 0.848. Thirteen of the loci were not in Hardy-Weinberg equilibrium, due to either the presence of null alleles, small sample size, or the effects of population subdivision (Wahlund's effect). All 37 primer pairs were tested with P. graminis and P. triticina showing that they are specific to P. coronata.

Original languageEnglish (US)
Pages (from-to)1395-1398
Number of pages4
JournalMolecular Ecology Resources
Volume8
Issue number6
DOIs
StatePublished - Nov 1 2008

Keywords

  • Microsatellite
  • Oat crown rust
  • Population structure
  • Puccinia coronata
  • Puccinia pathway

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