Development and characterization of microsatellite loci in dioecious figs (Ficus, Moraceae)

Annika M Moe, George D Weiblen

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Premise of the study: Microsatellite markers for dioecious fi g species, Ficus bernaysii King (section Sycocarpus), Ficus pachyrrhachis K. Schum. & Lauterb. (section Sycocarpus), and Ficus copiosa Steud. (section Sycidium) were developed to estimate gene flow among closely related fig species. Methods and Results: Loci were developed from tri- and tetranucleotide-enriched genomic libraries. The six most repeatable and scorable loci for F. bernaysii and F. pachyrrhachis were characterized in 50 individuals each of five species from section Sycocarpus. Four primer pairs from F. copiosa were screened in 50 individuals each of two species from section Sycidium. Observed heterozygosity ranged from 0.53 to 0.93 in loci developed from F. bernaysii, 0.25 to 0.86 in loci developed from F. pachyrrhachis, and 0.68 to 0.87 in loci developed from F. copiosa. Conclusions: Amplification across the two Ficus sections was observed in seven of the ten total primer pairs.

Original languageEnglish (US)
JournalAmerican Journal of Botany
Volume98
Issue number2
DOIs
StatePublished - Feb 1 2011

Fingerprint

Moraceae
Ficus
figs
Guarea
Microsatellite Repeats
microsatellite repeats
loci
heterozygosity
Gene Flow
Genomic Library
gene flow
genomic libraries
amplification
genomics

Keywords

  • Ficus
  • Microsatellites
  • New Guinea
  • Sycidium
  • Sycocarpus

Cite this

Development and characterization of microsatellite loci in dioecious figs (Ficus, Moraceae). / Moe, Annika M; Weiblen, George D.

In: American Journal of Botany, Vol. 98, No. 2, 01.02.2011.

Research output: Contribution to journalArticle

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