Developing genomic platforms for Chinese hamster ovary cells

Anne Kantardjieff, Peter Morin Nissom, Song Hui Chuah, Faraaz Yusufi, Nitya M. Jacob, Bhanu Chandra Mulukutla, Miranda Yap, Wei Shou Hu

Research output: Contribution to journalReview articlepeer-review

49 Scopus citations

Abstract

Chinese hamster ovary (CHO) cells are widely used in recombinant protein production, yet despite their importance in bioprocessing, few genomic resources have been developed for this cell line. Over the past several years, we have made considerable progress in the development of genomic tools for CHO. Using Sanger-based sequencing technology, we have accrued a sequence repertoire of more than 68,000 expressed sequence tags (ESTs), representing more than 28,000 unique CHO transcripts. Using closely related species, we have functionally annotated this sequence set and have currently achieved significant representation in a number of functional classes, including some closely tied to recombinant protein production. This sequence repository has been used to design custom CHO Affymetrix arrays for transcriptome analysis. Illumina Solexa deep sequencing technology was also applied to study the CHO cell transcriptome and survey the identity and expression of small RNAs. These applications demonstrate the utility of genomic tools, and illustrate the applicability of emerging next-generation sequencing technologies.

Original languageEnglish (US)
Pages (from-to)1028-1035
Number of pages8
JournalBiotechnology Advances
Volume27
Issue number6
DOIs
StatePublished - Nov 1 2009

Keywords

  • Chinese hamster ovary (CHO) cells
  • EST sequencing
  • Next-generation sequencing
  • Small RNA sequencing
  • Transcriptome analysis

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