DETERMINATION OF URINARY SUCCINYLACETONE BY CAPILLARY GAS CHROMATOGRAPHY.

Mendel Tuchman, Chester B. Whitley, Margaret L. Ramnaraine, Larry D. Bowers, Kelly D. Fregien, William Krivit

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

The average analytical recovery of succinylacetone added to urine and separated by capillary gas chromatography was 69% for solvent extraction and 72% for anion exchange separation. Treating succinylacetone with hydroxylamine hydrochloride at a pH of less than 5 caused formation of a derivative separated by capillary gas chromatography into two isomers: 3-methyl-5-isoxazole propionate and 5-methyl-3-isoxazole propionate as their trimethylsilyl derivatives (molecular weight 227). In a pH greater than or equal to 5, succinylacetone dioxime was formed and separated into 3 isomers as their trimethylsilyl derivatives (molecular weight 404).

Original languageEnglish (US)
Pages (from-to)211-215
Number of pages5
JournalJournal of Chromatographic Science
Volume22
Issue number5
DOIs
StatePublished - Jan 1 1984

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