Determination of urinary homovanillic and vanillylmandelic acids from dried filter paper samples: Assessment of potential methods for neuroblastoma screening

Mendel Tuchman, Christiane Auray-Blais, Margaret L.R. Ramnaraine, Joseph Neglia, William Krivit, Bernard Lemieux

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Urinary homovanillic (HVA) and vanillylmandelic (VMA) acids were analyzed on 200 random urine samples from patients with neuroblastoma and controls, after the samples had been dried onto absorbent filter paper. The acids were determined quantitatively by gas chromatography (GC) and qualitatively by thin layer chromatography (TLC). The results were analyzed for correlation between liquid urine samples and urine dried on filter paper and between TLC and GC methods. A high overall correlation for HVA and VMA (99%) was found between liquid and dried filter samples analyzed by GC. The correlations were more significant for samples with elevated levels of these acids than for those with normal levels. Normalization of the results to the urinary creatinine concentration (UCr) is indicated due to variations in urine concentration. Results from TLC analysis showed a false positive rate of 3.5% and a false negative rate of 0.5% compared to GC analysis. This work suggests that a combination of a sensitive TLC method with a rapid quantitative GC method would be suitable for mass neuroblastoma screening in infants.

Original languageEnglish (US)
Pages (from-to)173-177
Number of pages5
JournalClinical Biochemistry
Volume20
Issue number3
DOIs
StatePublished - Jun 1987

Bibliographical note

Funding Information:
This work was supported in part by N1H Grant No. T32-CA0944S and the Children's Cancer Research Fund of the University of Minnesota. We thank Dr. Charles Scriver for initiating the collaboration on neuroblastoma screening between the centers in Canada and the United States. We also thank Mr. Robert Gigubre and Mr. Michael Rosenberg for their technical assistance and Ms. Cyndi Launer for aiding with the statistical analysis.

Keywords

  • acids, urinary
  • catecholamines
  • chromatography, gas
  • chromatography, thin layer
  • homovanillic acid
  • neuroblastoma, screening
  • vanillylmandelic acid

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