Determination of Uric Acid in Canine Serum and Urine By High Performance Liquid Chromatography

Lawrence J. Felice, Dzintra Dombrovskis, Liz LaFond, Joseph Bartges, Carl A Osborne

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

A reproducible high performance liquid chromatography (HPLC) method was developed for analysis of uric acid in canine serum and urine. The method consists of precipitating serum proteins with phosphotungstic acid prior to HPLC analysis. Urine is analyzed after dilution with buffer. Chromatography is performed on a reversed‐phase C‐18 column with UV detection at 292 nm. Sensitivity of the method will allow reproducible measurement of uric acid at concentrations of 0.05 mg/dl in serum and 0.1 mg/dl in urine. The HPLC method has been used to quantify hundreds of canine serum and urine samples. The method is superior to UV absorption or colorimetric methods because its lower limit of detection allows measurement of uric acid at concentrations found in canine serum and urine.

Original languageEnglish (US)
Pages (from-to)86-89
Number of pages4
JournalVeterinary Clinical Pathology
Volume19
Issue number3
DOIs
StatePublished - Sep 1990

Keywords

  • canine
  • liquid chromatography
  • serum
  • uric acid
  • urine

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