Determination of trazodone and its metabolite, 1-m-chlorophenyl- piperazine, in human plasma and red blood cell samples by HPLC

Govind T. Vatassery, Lori A. Holden, Dana K. Hazel, Maurice W Dysken

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Objectives: To develop an HPLC method for the analysis of trazodone and its metabolite, 1-m-chlorophenyl-piperazine (m-CPP), in human plasma and red blood cells. Design and Methods: The analytes were extracted by heptane containing 1.5% isoamyl alcohol, back extracted into phosphoric acid and analyzed by reverse phase HPLC with UV detection. Results: In seven randomly selected, male, human subjects plasma concentrations (nmol/l) were 380-5841 for trazodone and 14-237 for rn-CPP while those in packed red blood cells were 98-634 for trazodone and 15-155 for m-CPP. Plasma trazodone concentrations were 4-11-fold higher than those in red blood cells while those of m-CPP were about equal. This may be the first report on concentrations of trazodone and m-CPP in human red blood cells. Conclusions: This sensitive method can be used for monitoring trazodone and m-CPP in human plasma and red blood cells.

Original languageEnglish (US)
Pages (from-to)149-153
Number of pages5
JournalClinical Biochemistry
Volume30
Issue number2
DOIs
StatePublished - Mar 1 1997

Keywords

  • and erythrocytes
  • antidepressive agents
  • high pressure liquid chromatography
  • piperazines
  • plasma
  • trazodone

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