Determination of the sensitivity and specificity of bovine tuberculosis screening tests in dairy herds in Thailand using a Bayesian approach

Tawatchai Singhla, Sukolrat Boonyayatra, Songkhla Chulakasian, Mintra Lukkana, Julio Alvarez, Srinand Sreevatsan, Scott J Wells

Research output: Contribution to journalArticle

Abstract

Background: The objective of this study was to determine the sensitivity (Se) and specificity (Sp) of bovine tuberculosis (bTB) screening tests including a single intradermal tuberculin (SIT) test, interferon gamma (IFN-γ) assay, and a commercial ELISA test (M. bovis Ab) in dairy cattle, under field conditions, using a Bayesian approach. Results: The study population consisted of 128 dairy cows from 25 bTB-infected herds in Chiang Mai and Chiang Rai provinces, Thailand. A single-population Bayesian model was implemented assuming conditional dependence between the SIT test and IFN-γassays. The 95% posterior probability interval (PPI) of the SIT test (severe interpretation) Se ranged from 75.3 to 95.2% (median = 87.6%), while the Sp was slightly lower (median = 83.6%, PPI = 74.2-92.8%). The IFN-γassay Se was moderate and the 95% PPI ranged from 38.6 to 74.4% (median = 55.7%) with higher Sp (median = 93.5.4%, PPI = 87.0-98.1%). The M. bovis Ab ELISA Se was low, with 95% PPI ranging between 30.0 and 71.2% (median = 47.4%); however, the Sp was high (median = 90.9%, PPI = 84.5-95.5%). Conclusion: The SIT test sensitivity was similar to that demonstrated in other regions and can, therefore, be used effectively as part of control programs in this area. The IFN-γand M. bovis Ab ELISA assays can be applied as supplementary techniques. The test performance of these tests when used as single tests without confirmation, however, are expected to continue to challenge disease eradication efforts.

Original languageEnglish (US)
Article number149
JournalBMC Veterinary Research
Volume15
Issue number1
DOIs
StatePublished - May 16 2019

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Bovine Tuberculosis
Bayes Theorem
bovine tuberculosis
Thailand
dairy herds
Intradermal Tests
Tuberculin Test
screening
Sensitivity and Specificity
Interferon-gamma
tuberculin
interferon-gamma
Enzyme-Linked Immunosorbent Assay
testing
enzyme-linked immunosorbent assay
assays
Disease Eradication
Population
dairy cattle
dairy cows

Keywords

  • ELISA test
  • Interferon gamma assay
  • Mycobacterium bovis
  • Single intradermal tuberculin test
  • Test performance

Cite this

Determination of the sensitivity and specificity of bovine tuberculosis screening tests in dairy herds in Thailand using a Bayesian approach. / Singhla, Tawatchai; Boonyayatra, Sukolrat; Chulakasian, Songkhla; Lukkana, Mintra; Alvarez, Julio; Sreevatsan, Srinand; Wells, Scott J.

In: BMC Veterinary Research, Vol. 15, No. 1, 149, 16.05.2019.

Research output: Contribution to journalArticle

Singhla, Tawatchai ; Boonyayatra, Sukolrat ; Chulakasian, Songkhla ; Lukkana, Mintra ; Alvarez, Julio ; Sreevatsan, Srinand ; Wells, Scott J. / Determination of the sensitivity and specificity of bovine tuberculosis screening tests in dairy herds in Thailand using a Bayesian approach. In: BMC Veterinary Research. 2019 ; Vol. 15, No. 1.
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abstract = "Background: The objective of this study was to determine the sensitivity (Se) and specificity (Sp) of bovine tuberculosis (bTB) screening tests including a single intradermal tuberculin (SIT) test, interferon gamma (IFN-γ) assay, and a commercial ELISA test (M. bovis Ab) in dairy cattle, under field conditions, using a Bayesian approach. Results: The study population consisted of 128 dairy cows from 25 bTB-infected herds in Chiang Mai and Chiang Rai provinces, Thailand. A single-population Bayesian model was implemented assuming conditional dependence between the SIT test and IFN-γassays. The 95{\%} posterior probability interval (PPI) of the SIT test (severe interpretation) Se ranged from 75.3 to 95.2{\%} (median = 87.6{\%}), while the Sp was slightly lower (median = 83.6{\%}, PPI = 74.2-92.8{\%}). The IFN-γassay Se was moderate and the 95{\%} PPI ranged from 38.6 to 74.4{\%} (median = 55.7{\%}) with higher Sp (median = 93.5.4{\%}, PPI = 87.0-98.1{\%}). The M. bovis Ab ELISA Se was low, with 95{\%} PPI ranging between 30.0 and 71.2{\%} (median = 47.4{\%}); however, the Sp was high (median = 90.9{\%}, PPI = 84.5-95.5{\%}). Conclusion: The SIT test sensitivity was similar to that demonstrated in other regions and can, therefore, be used effectively as part of control programs in this area. The IFN-γand M. bovis Ab ELISA assays can be applied as supplementary techniques. The test performance of these tests when used as single tests without confirmation, however, are expected to continue to challenge disease eradication efforts.",
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AU - Singhla, Tawatchai

AU - Boonyayatra, Sukolrat

AU - Chulakasian, Songkhla

AU - Lukkana, Mintra

AU - Alvarez, Julio

AU - Sreevatsan, Srinand

AU - Wells, Scott J

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AB - Background: The objective of this study was to determine the sensitivity (Se) and specificity (Sp) of bovine tuberculosis (bTB) screening tests including a single intradermal tuberculin (SIT) test, interferon gamma (IFN-γ) assay, and a commercial ELISA test (M. bovis Ab) in dairy cattle, under field conditions, using a Bayesian approach. Results: The study population consisted of 128 dairy cows from 25 bTB-infected herds in Chiang Mai and Chiang Rai provinces, Thailand. A single-population Bayesian model was implemented assuming conditional dependence between the SIT test and IFN-γassays. The 95% posterior probability interval (PPI) of the SIT test (severe interpretation) Se ranged from 75.3 to 95.2% (median = 87.6%), while the Sp was slightly lower (median = 83.6%, PPI = 74.2-92.8%). The IFN-γassay Se was moderate and the 95% PPI ranged from 38.6 to 74.4% (median = 55.7%) with higher Sp (median = 93.5.4%, PPI = 87.0-98.1%). The M. bovis Ab ELISA Se was low, with 95% PPI ranging between 30.0 and 71.2% (median = 47.4%); however, the Sp was high (median = 90.9%, PPI = 84.5-95.5%). Conclusion: The SIT test sensitivity was similar to that demonstrated in other regions and can, therefore, be used effectively as part of control programs in this area. The IFN-γand M. bovis Ab ELISA assays can be applied as supplementary techniques. The test performance of these tests when used as single tests without confirmation, however, are expected to continue to challenge disease eradication efforts.

KW - ELISA test

KW - Interferon gamma assay

KW - Mycobacterium bovis

KW - Single intradermal tuberculin test

KW - Test performance

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