Determination of the quaternary structure of protocatechuate 3,4-dioxygenase from Pseudomonas aeruginosa

D. H. Ohlendorf; P. C. Weber; J. D. Lipscomb

doi:10.1016/0022-2836(87)90340-8

Determination of the quaternary structure of protocatechuate 3,4-dioxygenase from Pseudomonas aeruginosa

D. H. Ohlendorf, P. C. Weber, J. D. Lipscomb

Biochemistry, Molecular Biology, and Biophysics (TMED)

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

A 2.5 Å resolution data set has been collected for crystals of protocatechuate 3,4-dioxygenase from Pseudomonas aeruginosa. Analysis of the data using the rotation function shows that the α₂β₂ tetramers associate to form a particle with cubic 23 (T) point group symmetry. Prior to this analysis it was believed that eight tetramers associated to form the holoenzyme. The symmetry of the crystalline holoenzyme also addresses questions concerning its iron content and substrate stoichiometry.

Original language	English (US)
Pages (from-to)	225-227
Number of pages	3
Journal	Journal of Molecular Biology
Volume	195
Issue number	1
DOIs	https://doi.org/10.1016/0022-2836(87)90340-8
State	Published - May 5 1987

Bibliographical note

Funding Information:
We thank the Genex Corporation, Gaithersburg, MD for use of the Xentronics data collection facility and acknowledge the technical assistance of A. Orville and W. Froland. This work was supported by NIGMS GM24689 to one of us (J.D.L.).

Publisher link

10.1016/0022-2836(87)90340-8

Find It

Find It at U of M Libraries

Cite this

@article{3880f0e06d2e4f578260ec5ec149f805,

title = "Determination of the quaternary structure of protocatechuate 3,4-dioxygenase from Pseudomonas aeruginosa",

abstract = "A 2.5 {\AA} resolution data set has been collected for crystals of protocatechuate 3,4-dioxygenase from Pseudomonas aeruginosa. Analysis of the data using the rotation function shows that the α2β2 tetramers associate to form a particle with cubic 23 (T) point group symmetry. Prior to this analysis it was believed that eight tetramers associated to form the holoenzyme. The symmetry of the crystalline holoenzyme also addresses questions concerning its iron content and substrate stoichiometry.",

author = "Ohlendorf, {D. H.} and Weber, {P. C.} and Lipscomb, {J. D.}",

note = "Funding Information: We thank the Genex Corporation, Gaithersburg, MD for use of the Xentronics data collection facility and acknowledge the technical assistance of A. Orville and W. Froland. This work was supported by NIGMS GM24689 to one of us (J.D.L.).",

year = "1987",

month = may,

day = "5",

doi = "10.1016/0022-2836(87)90340-8",

language = "English (US)",

volume = "195",

pages = "225--227",

journal = "Journal of Molecular Biology",

issn = "0022-2836",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Determination of the quaternary structure of protocatechuate 3,4-dioxygenase from Pseudomonas aeruginosa

AU - Ohlendorf, D. H.

AU - Weber, P. C.

AU - Lipscomb, J. D.

N1 - Funding Information: We thank the Genex Corporation, Gaithersburg, MD for use of the Xentronics data collection facility and acknowledge the technical assistance of A. Orville and W. Froland. This work was supported by NIGMS GM24689 to one of us (J.D.L.).

PY - 1987/5/5

Y1 - 1987/5/5

N2 - A 2.5 Å resolution data set has been collected for crystals of protocatechuate 3,4-dioxygenase from Pseudomonas aeruginosa. Analysis of the data using the rotation function shows that the α2β2 tetramers associate to form a particle with cubic 23 (T) point group symmetry. Prior to this analysis it was believed that eight tetramers associated to form the holoenzyme. The symmetry of the crystalline holoenzyme also addresses questions concerning its iron content and substrate stoichiometry.

AB - A 2.5 Å resolution data set has been collected for crystals of protocatechuate 3,4-dioxygenase from Pseudomonas aeruginosa. Analysis of the data using the rotation function shows that the α2β2 tetramers associate to form a particle with cubic 23 (T) point group symmetry. Prior to this analysis it was believed that eight tetramers associated to form the holoenzyme. The symmetry of the crystalline holoenzyme also addresses questions concerning its iron content and substrate stoichiometry.

UR - http://www.scopus.com/inward/record.url?scp=0023644834&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023644834&partnerID=8YFLogxK

U2 - 10.1016/0022-2836(87)90340-8

DO - 10.1016/0022-2836(87)90340-8

M3 - Article

C2 - 3116260

AN - SCOPUS:0023644834

SN - 0022-2836

VL - 195

SP - 225

EP - 227

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 1

ER -

Determination of the quaternary structure of protocatechuate 3,4-dioxygenase from Pseudomonas aeruginosa

Abstract

Bibliographical note

Publisher link

Find It

Other files and links

Fingerprint

Cite this