Determination of the quaternary structure of protocatechuate 3,4-dioxygenase from Pseudomonas aeruginosa

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Abstract

A 2.5 Å resolution data set has been collected for crystals of protocatechuate 3,4-dioxygenase from Pseudomonas aeruginosa. Analysis of the data using the rotation function shows that the α2β2 tetramers associate to form a particle with cubic 23 (T) point group symmetry. Prior to this analysis it was believed that eight tetramers associated to form the holoenzyme. The symmetry of the crystalline holoenzyme also addresses questions concerning its iron content and substrate stoichiometry.

Original languageEnglish (US)
Pages (from-to)225-227
Number of pages3
JournalJournal of Molecular Biology
Volume195
Issue number1
DOIs
StatePublished - May 5 1987

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Protocatechuate-3,4-Dioxygenase
Holoenzymes
Pseudomonas aeruginosa
Iron

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Determination of the quaternary structure of protocatechuate 3,4-dioxygenase from Pseudomonas aeruginosa. / Ohlendorf, D. H.; Weber, P. C.; Lipscomb, J. D.

In: Journal of Molecular Biology, Vol. 195, No. 1, 05.05.1987, p. 225-227.

Research output: Contribution to journalArticle

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