Determination of proteins by amperometric titration with 12-phosphotungstic acid at rotating gold electrodes

Stephen R. Betso, Peter W. Carr

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Amperometric titration of various protein fractions and human serum with 12-phosphotungstic acid at the rotating gold electrode is described. The titrant is electroreducible and its concentration may be monitored by poising an amperometric indicator electrode at a potential of -0.25 V vs. SCE. In 1.0 M hydrochloric acid, well defined titration curves of proteins may be obtained in the presence of airsaturated solutions. The relative standard deviation of replicate samples is 0.2-0.3% in the range 2-4 g of protein per 1 in the sample solution. The technique is principally applicable to homogeneous mixtures of proteins.

Original languageEnglish (US)
Pages (from-to)161-172
Number of pages12
JournalAnalytica Chimica Acta
Volume69
Issue number1
DOIs
StatePublished - Mar 1974

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