TY - JOUR
T1 - Determination of Protein Interaction in Milk Protein Concentrate Powders Manufactured from pH-Adjusted and Heat-Treated Skim Milk
AU - Dileep, Kavya
AU - Meletharayil, Hari
AU - Amamcharla, Jayendra K.
N1 - Publisher Copyright:
© 2024 by the authors.
PY - 2024/12
Y1 - 2024/12
N2 - The influence of heating as a pretreatment on the structural and functional attributes of milk protein concentrate (MPC) powders derived from ultrafiltered/diafiltered (UF/DF) skim milk is under-reported. This research delves into the impact of pH and heat treatment on skim milk’s properties before UF/DF and how these changes affect the resulting MPC powders. By adjusting the pH of skim milk to 6.5, 6.8, or 7.1 and applying thermal treatment at 90 °C for 15 min to one of two divided lots (with the other serving as a control), we studied the protein interactions in MPC. Post-heat treatment, the skim milk’s pH was adjusted back to 6.8, followed by ultrafiltration and spray drying to produce MPC powders with protein content of 82.38 ± 2.72% on a dry matter basis. MPC dispersions from these powders at 5% protein (w/w) were also evaluated for particle size, viscosity, and heat coagulation time (HCT) to further understand how the protein interactions in skim milk influence the properties of MPC dispersions. Capillary electrophoresis was used to assess the casein and whey protein distribution in both the soluble and colloidal phases. Findings revealed that preheating skim milk at pH 7.1 increased serum phase interactions, while heating skim milk preadjusted to a pH of 6.5 promoted whey protein–casein interactions at the micellar interface. Notably, the D (4,3) of casein micelles was larger for dispersions from milk with a preheated pH of 6.5 compared to other pH levels, correlating positively with enhanced dispersion viscosity due to increased volume fraction. These results support the potential for tailoring MPC powder functionality in various food applications through the precise control of the milk’s pre-treatment conditions.
AB - The influence of heating as a pretreatment on the structural and functional attributes of milk protein concentrate (MPC) powders derived from ultrafiltered/diafiltered (UF/DF) skim milk is under-reported. This research delves into the impact of pH and heat treatment on skim milk’s properties before UF/DF and how these changes affect the resulting MPC powders. By adjusting the pH of skim milk to 6.5, 6.8, or 7.1 and applying thermal treatment at 90 °C for 15 min to one of two divided lots (with the other serving as a control), we studied the protein interactions in MPC. Post-heat treatment, the skim milk’s pH was adjusted back to 6.8, followed by ultrafiltration and spray drying to produce MPC powders with protein content of 82.38 ± 2.72% on a dry matter basis. MPC dispersions from these powders at 5% protein (w/w) were also evaluated for particle size, viscosity, and heat coagulation time (HCT) to further understand how the protein interactions in skim milk influence the properties of MPC dispersions. Capillary electrophoresis was used to assess the casein and whey protein distribution in both the soluble and colloidal phases. Findings revealed that preheating skim milk at pH 7.1 increased serum phase interactions, while heating skim milk preadjusted to a pH of 6.5 promoted whey protein–casein interactions at the micellar interface. Notably, the D (4,3) of casein micelles was larger for dispersions from milk with a preheated pH of 6.5 compared to other pH levels, correlating positively with enhanced dispersion viscosity due to increased volume fraction. These results support the potential for tailoring MPC powder functionality in various food applications through the precise control of the milk’s pre-treatment conditions.
KW - caseins
KW - heating
KW - MPC powders
KW - pH
KW - physicochemical properties
KW - whey protein
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U2 - 10.3390/foods13233832
DO - 10.3390/foods13233832
M3 - Article
C2 - 39682904
AN - SCOPUS:85211784532
SN - 2304-8158
VL - 13
JO - Foods
JF - Foods
IS - 23
M1 - 3832
ER -