Determination of metabolic stability using cryopreserved hepatocytes from rainbow trout (Oncorhynchus mykiss)

Kellie A. Fay, Diane L. Nabb, Robert T. Mingoia, Ina Bischof, John W. Nichols, Helmut Segner, Karla Johanning, Xing Han

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Trout provide a relatively easy source of hepatocytes that can be cryopreserved and used for a range of applications including toxicity testing and determination of intrinsic clearance. Standard protocols for isolating, cryopreserving, and thawing rainbow trout hepatocytes are described, along with procedures for using fresh or cryopreserved hepatocytes to assess metabolic stability of xenobiotics in fish by means of a substrate depletion approach. Variations on these methods, troubleshooting tips, and directions for use of extrapolation factors to express results in terms of in vivo intrinsic clearance are included. These protocols have been developed for rainbow trout, but can be adapted to other fish species with appropriate considerations.

Original languageEnglish (US)
Pages (from-to)4.42.1-4.42.29
JournalCurrent protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.]
Volume2015
DOIs
StatePublished - 2015

Keywords

  • Biotransformation
  • Fish
  • Hepatocytes
  • In vitro assay
  • Metabolic stability
  • Rainbow trout
  • Substrate depletion

PubMed: MeSH publication types

  • Journal Article

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