Determination of Internucleotide h J HN Couplings by the Modified 2D J NN -Correlated [ 15 N, 1 H] TROSY

Xianzhong Yan, Xiangming Kong, Youlin Xia, Kong Hung Sze, Guang Zhu

Research output: Contribution to journalEditorialpeer-review

10 Scopus citations


Recent developments in the direct observation of J couplings across hydrogen bonds in proteins and nucleic acids provide additional information for structure and function studies of these molecules by NMR spectroscopy. A J NN -correlated [ 15 N, 1 H] TROSY experiment proposed by Pervushin et al. (Proc. Natl. Acad. Sci. USA 95, 14147-14151, 1998) can be applied to measure h J HN in smaller nucleic acids in an E.COSY manner. However, it cannot be effectively applied to large nucleic acids, such as tRNA Trp , since one of the peaks corresponding to a fast relaxing component will be too weak to be observed in the spectra of large molecules. In this Communication, we proposed a modified J NN -correlated [ 15 N, 1 H] TROSY experiment which enables direct measurement of h J HN in large nucleic acids.

Original languageEnglish (US)
Pages (from-to)357-360
Number of pages4
JournalJournal of Magnetic Resonance
Issue number2
StatePublished - Dec 2000


  • Hydrogen bond
  • J -correlated [ N, H] TROSY
  • NMR
  • tRNA

Fingerprint Dive into the research topics of 'Determination of Internucleotide <sup>h</sup> J <sub>HN</sub> Couplings by the Modified 2D J <sub>NN</sub> -Correlated [ <sup>15</sup> N, <sup>1</sup> H] TROSY'. Together they form a unique fingerprint.

Cite this