Determination of Internucleotide h J HN Couplings by the Modified 2D J NN -Correlated [ 15 N, 1 H] TROSY

Xianzhong Yan, Xiangming Kong, Youlin Xia, Kong Hung Sze, Guang Zhu

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12 Scopus citations

Abstract

Recent developments in the direct observation of J couplings across hydrogen bonds in proteins and nucleic acids provide additional information for structure and function studies of these molecules by NMR spectroscopy. A J NN -correlated [ 15 N, 1 H] TROSY experiment proposed by Pervushin et al. (Proc. Natl. Acad. Sci. USA 95, 14147-14151, 1998) can be applied to measure h J HN in smaller nucleic acids in an E.COSY manner. However, it cannot be effectively applied to large nucleic acids, such as tRNA Trp , since one of the peaks corresponding to a fast relaxing component will be too weak to be observed in the spectra of large molecules. In this Communication, we proposed a modified J NN -correlated [ 15 N, 1 H] TROSY experiment which enables direct measurement of h J HN in large nucleic acids.

Original languageEnglish (US)
Pages (from-to)357-360
Number of pages4
JournalJournal of Magnetic Resonance
Volume147
Issue number2
DOIs
StatePublished - Dec 2000
Externally publishedYes

Bibliographical note

Funding Information:
This work is supported by grants from the Research Grant Council of Hong Kong (HKUST6197/97M, HKUST6038/98M, and HKUST6199/99M). The Hong Kong Biotechnology Research Institute is acknowledged for the purchase of the 750-MHz NMR spectrometer. The authors also thank Dr. H. Xue and Prof. J. Wong for their help with sample preparation.

Keywords

  • Hydrogen bond
  • J -correlated [ N, H] TROSY
  • NMR
  • TROSY
  • tRNA

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