Abstract
A method is described for the determination of glutamate in superfusates of cortical or hippocampal rat brain slices. This method is based on the precolumn derivatization of amino acids with orthopthaldialdehyde and mercaptopropionic acid, separation by high-performance liquid chromatography, and detection by fluorescence. By adjusting the concentrations of reagents and with the minimum dilution of the sample, it was possible to reproducibly measure the Ca2+-dependent K+-evoked release of neurotransmitter glutamate in superfusates of brain slices.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 82-86 |
| Number of pages | 5 |
| Journal | Analytical Biochemistry |
| Volume | 264 |
| Issue number | 1 |
| DOIs | |
| State | Published - Nov 1 1998 |
| Externally published | Yes |
Bibliographical note
Funding Information:This work was supported by Grant 70/3/2946 from the Greek General Secretariat of Research and Technology to George Palaio-logos and the University of Athens. We are grateful to Professor C. E. Sekeris, Chairman of the Department of Biological Chemistry of the Medical School, University of Athens, for his support. Part of this work was presented at the 11th Balkan Biochemical and Biophysical Days at Thessaloniki, Greece, May 15–17, 1997.