Determination of DNA Hybridization on Gold Nanoparticle Conjugated Polystyrene Particle Thin Film Using Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy

Juan Otano; Minh Phuong N Bui; Seong S. Seo

doi:10.1080/00032719.2013.831429

Determination of DNA Hybridization on Gold Nanoparticle Conjugated Polystyrene Particle Thin Film Using Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy

Juan Otano, Minh Phuong N Bui, Seong S. Seo

Bioproducts and Biosystems Engineering

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Attenuated total reflectance Fourier transform infrared spectroscopy was used to detect DNA hybridization on a polystyrene conjugated gold nanoparticle thin film. The gold nanoparticles were synthesized on the surface of poly(ethylenimine) coated polystyrene particles by citrate reduction. Single-stranded DNA was then immobilized on the nanoparticle surface via thiol bonding. Ultraviolet-visible spectrometry was used to monitor the conjugation of the nanoparticles on polystyrene particles and the immobilization of a single-stranded DNA probe. The morphology of the polystyrene-gold nanoparticle thin film was characterized using scanning electron microscopy and showed successful conjugation and immobilization. The infrared spectra obtained from the hybridization showed features of DNA structure and peak shifts at 1657 cm^-1 compared to the non-complementary DNA due to changes in hydrogen bonding between N-H and C=O of complimentary bases pairs. The peaks at 1067, 975, 920, and 859 cm^-1, which were shifted to lower wavenumbers in the polystyrene-gold nanoparticle probe and target DNA, indicated hydrogen bonding formation between N-H and N of complimentary base pairs. ATR-FTIR spectroscopy provided simple, fast, and portable label-free detection of target DNA sequence on the polystyrene-gold nanoparticle thin film.

Original language	English (US)
Pages (from-to)	167-177
Number of pages	11
Journal	Analytical Letters
Volume	47
Issue number	1
DOIs	https://doi.org/10.1080/00032719.2013.831429
State	Published - Jan 2014

Bibliographical note

Funding Information:
Received 24 May 2013; accepted 2 July 2013. J. Otano and M.-P. N. Bui contributed equally. This work was supported by grants from the Department of Defense, Grant # W911NF-11-1-0181, DOD CDMRP Training Grant: Grant # W81XWH-10-1-1042 and the undergraduate research program at Albany State University whose support is greatly appreciated. Address correspondence to Dr. Seong S. Seo, Department of Natural Science, Albany State University, Albany, GA 31705, USA. E-mail: [email protected]

Keywords

ATR-FTIR
DNA hybridization
Gold nanoparticle
Polystyrene
Thin film

Publisher link

10.1080/00032719.2013.831429

Find It

Find It at U of M Libraries

Cite this

@article{19c8eec5456245fb8533c9df97b53c94,

title = "Determination of DNA Hybridization on Gold Nanoparticle Conjugated Polystyrene Particle Thin Film Using Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy",

abstract = "Attenuated total reflectance Fourier transform infrared spectroscopy was used to detect DNA hybridization on a polystyrene conjugated gold nanoparticle thin film. The gold nanoparticles were synthesized on the surface of poly(ethylenimine) coated polystyrene particles by citrate reduction. Single-stranded DNA was then immobilized on the nanoparticle surface via thiol bonding. Ultraviolet-visible spectrometry was used to monitor the conjugation of the nanoparticles on polystyrene particles and the immobilization of a single-stranded DNA probe. The morphology of the polystyrene-gold nanoparticle thin film was characterized using scanning electron microscopy and showed successful conjugation and immobilization. The infrared spectra obtained from the hybridization showed features of DNA structure and peak shifts at 1657 cm-1 compared to the non-complementary DNA due to changes in hydrogen bonding between N-H and C=O of complimentary bases pairs. The peaks at 1067, 975, 920, and 859 cm-1, which were shifted to lower wavenumbers in the polystyrene-gold nanoparticle probe and target DNA, indicated hydrogen bonding formation between N-H and N of complimentary base pairs. ATR-FTIR spectroscopy provided simple, fast, and portable label-free detection of target DNA sequence on the polystyrene-gold nanoparticle thin film.",

keywords = "ATR-FTIR, DNA hybridization, Gold nanoparticle, Polystyrene, Thin film",

author = "Juan Otano and Bui, {Minh Phuong N} and Seo, {Seong S.}",

note = "Funding Information: Received 24 May 2013; accepted 2 July 2013. J. Otano and M.-P. N. Bui contributed equally. This work was supported by grants from the Department of Defense, Grant # W911NF-11-1-0181, DOD CDMRP Training Grant: Grant # W81XWH-10-1-1042 and the undergraduate research program at Albany State University whose support is greatly appreciated. Address correspondence to Dr. Seong S. Seo, Department of Natural Science, Albany State University, Albany, GA 31705, USA. E-mail: [email protected]",

year = "2014",

month = jan,

doi = "10.1080/00032719.2013.831429",

language = "English (US)",

volume = "47",

pages = "167--177",

journal = "Analytical Letters",

issn = "0003-2719",

publisher = "Taylor and Francis Ltd.",

number = "1",

}

TY - JOUR

T1 - Determination of DNA Hybridization on Gold Nanoparticle Conjugated Polystyrene Particle Thin Film Using Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy

AU - Otano, Juan

AU - Bui, Minh Phuong N

AU - Seo, Seong S.

N1 - Funding Information: Received 24 May 2013; accepted 2 July 2013. J. Otano and M.-P. N. Bui contributed equally. This work was supported by grants from the Department of Defense, Grant # W911NF-11-1-0181, DOD CDMRP Training Grant: Grant # W81XWH-10-1-1042 and the undergraduate research program at Albany State University whose support is greatly appreciated. Address correspondence to Dr. Seong S. Seo, Department of Natural Science, Albany State University, Albany, GA 31705, USA. E-mail: [email protected]

PY - 2014/1

Y1 - 2014/1

N2 - Attenuated total reflectance Fourier transform infrared spectroscopy was used to detect DNA hybridization on a polystyrene conjugated gold nanoparticle thin film. The gold nanoparticles were synthesized on the surface of poly(ethylenimine) coated polystyrene particles by citrate reduction. Single-stranded DNA was then immobilized on the nanoparticle surface via thiol bonding. Ultraviolet-visible spectrometry was used to monitor the conjugation of the nanoparticles on polystyrene particles and the immobilization of a single-stranded DNA probe. The morphology of the polystyrene-gold nanoparticle thin film was characterized using scanning electron microscopy and showed successful conjugation and immobilization. The infrared spectra obtained from the hybridization showed features of DNA structure and peak shifts at 1657 cm-1 compared to the non-complementary DNA due to changes in hydrogen bonding between N-H and C=O of complimentary bases pairs. The peaks at 1067, 975, 920, and 859 cm-1, which were shifted to lower wavenumbers in the polystyrene-gold nanoparticle probe and target DNA, indicated hydrogen bonding formation between N-H and N of complimentary base pairs. ATR-FTIR spectroscopy provided simple, fast, and portable label-free detection of target DNA sequence on the polystyrene-gold nanoparticle thin film.

AB - Attenuated total reflectance Fourier transform infrared spectroscopy was used to detect DNA hybridization on a polystyrene conjugated gold nanoparticle thin film. The gold nanoparticles were synthesized on the surface of poly(ethylenimine) coated polystyrene particles by citrate reduction. Single-stranded DNA was then immobilized on the nanoparticle surface via thiol bonding. Ultraviolet-visible spectrometry was used to monitor the conjugation of the nanoparticles on polystyrene particles and the immobilization of a single-stranded DNA probe. The morphology of the polystyrene-gold nanoparticle thin film was characterized using scanning electron microscopy and showed successful conjugation and immobilization. The infrared spectra obtained from the hybridization showed features of DNA structure and peak shifts at 1657 cm-1 compared to the non-complementary DNA due to changes in hydrogen bonding between N-H and C=O of complimentary bases pairs. The peaks at 1067, 975, 920, and 859 cm-1, which were shifted to lower wavenumbers in the polystyrene-gold nanoparticle probe and target DNA, indicated hydrogen bonding formation between N-H and N of complimentary base pairs. ATR-FTIR spectroscopy provided simple, fast, and portable label-free detection of target DNA sequence on the polystyrene-gold nanoparticle thin film.

KW - ATR-FTIR

KW - DNA hybridization

KW - Gold nanoparticle

KW - Polystyrene

KW - Thin film

UR - http://www.scopus.com/inward/record.url?scp=84891339920&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84891339920&partnerID=8YFLogxK

U2 - 10.1080/00032719.2013.831429

DO - 10.1080/00032719.2013.831429

M3 - Article

AN - SCOPUS:84891339920

SN - 0003-2719

VL - 47

SP - 167

EP - 177

JO - Analytical Letters

JF - Analytical Letters

IS - 1

ER -

Determination of DNA Hybridization on Gold Nanoparticle Conjugated Polystyrene Particle Thin Film Using Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy

Abstract

Bibliographical note

Keywords

Publisher link

Find It

Other files and links

Fingerprint

Cite this