Determination of deoxymyoglobin changes during graded myocardial ischemia: An in vivo 1H NMR spectroscopy study

Wei Chen, Jianyi Zhang, Marcel H.J. Eljgelshoven, Yi Zhang, Xiao Hong Zhu, Chunshen Wang, Yong Cho, Hellmut Merkle, Kâmil Uĝurbil

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Abstract

1H NMR spectroscopy was used to detect the proximal histidyl N(δ) proton signal of deoxymyoglobin from canine hearts in vivo during graded myocardial ischemia. The NMR signal intensity provided an indicator of intracellular oxygenation in myocardium. The relationship between the myocardial blood flow and the deoxymyoglobin concentration was successfully measured during resting, partial, and complete coronary artery occlusion conditions. The results demonstrate the feasibility to detect deoxymyoglobin using a 1H NMR spectroscopy technique in living hearts for the first time and the possibility to use this technique for investigating myocardial oxidative metabolism nondestructively and repetitively.

Original languageEnglish (US)
Pages (from-to)193-197
Number of pages5
JournalMagnetic resonance in medicine
Volume38
Issue number2
DOIs
StatePublished - Aug 1 1997

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Keywords

  • Deoxymyoglobin
  • H NMR spectroscopy
  • Myocardial ischemia
  • Oxygenation

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