Abstract
Tiletamine and zolazepam injection (Telazol®) is used in veterinary surgical practice to induce short-term anesthesia and also to immobilize wild animals. The present work describes a sensitive method to measure tiletamine and zolazepam concentrations in plasma by means of GC/EI-MS on a 5% phenyl/95% methylpolysiloxane column. A simple liquid extraction procedure with ethyl acetate was used to isolate the two compounds and the same were separated and analyzed by GC/MS without derivatization. A formal validation of the assay demonstrated good accuracy and precision for both tiletamine (98-100.8%; C.V.total < 6.7%) and zolazepam (98.3-103.4; C.V.total < 13.2%). With 500 μl of plasma, the limits of quantification for both tiletamine and zolazepam were found to be 10 ng/ml. Both compounds were stable after three freeze-thaw cycles. The assay was used to analyze plasma samples collected from a pig after intramuscular administration of 10 mg/kg of Telazol®. The plasma concentration-time profile of tiletamine and zolazepam from this representative pig is also provided.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 131-135 |
| Number of pages | 5 |
| Journal | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences |
| Volume | 842 |
| Issue number | 2 SPEC. ISS. |
| DOIs | |
| State | Published - Oct 2 2006 |
Bibliographical note
Funding Information:This work was supported in part by a grant from the Office of the Dean of the Graduate School of the University of Minnesota. The help of personnel at Experimental Surgical Services at University of Minnesota in collecting pig plasma samples is greatly appreciated.
Keywords
- GC/MS
- Pharmacokinetics
- Pig
- Tiletamine
- Zolazepam