Abstract
Tiletamine and zolazepam injection (Telazol®) is used in veterinary surgical practice to induce short-term anesthesia and also to immobilize wild animals. The present work describes a sensitive method to measure tiletamine and zolazepam concentrations in plasma by means of GC/EI-MS on a 5% phenyl/95% methylpolysiloxane column. A simple liquid extraction procedure with ethyl acetate was used to isolate the two compounds and the same were separated and analyzed by GC/MS without derivatization. A formal validation of the assay demonstrated good accuracy and precision for both tiletamine (98-100.8%; C.V.total < 6.7%) and zolazepam (98.3-103.4; C.V.total < 13.2%). With 500 μl of plasma, the limits of quantification for both tiletamine and zolazepam were found to be 10 ng/ml. Both compounds were stable after three freeze-thaw cycles. The assay was used to analyze plasma samples collected from a pig after intramuscular administration of 10 mg/kg of Telazol®. The plasma concentration-time profile of tiletamine and zolazepam from this representative pig is also provided.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 131-135 |
| Number of pages | 5 |
| Journal | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences |
| Volume | 842 |
| Issue number | 2 SPEC. ISS. |
| DOIs | |
| State | Published - Oct 2 2006 |
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Keywords
- GC/MS
- Pharmacokinetics
- Pig
- Tiletamine
- Zolazepam
Cite this
Determination of constituents of Telazol®-tiletamine and zolazepam by a gas chromatography/mass spectrometry-based method. / Kumar, Atul; Mann, Henry J.; Remmel, Rory P.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 842, No. 2 SPEC. ISS., 02.10.2006, p. 131-135.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Determination of constituents of Telazol®-tiletamine and zolazepam by a gas chromatography/mass spectrometry-based method
AU - Kumar, Atul
AU - Mann, Henry J.
AU - Remmel, Rory P
PY - 2006/10/2
Y1 - 2006/10/2
N2 - Tiletamine and zolazepam injection (Telazol®) is used in veterinary surgical practice to induce short-term anesthesia and also to immobilize wild animals. The present work describes a sensitive method to measure tiletamine and zolazepam concentrations in plasma by means of GC/EI-MS on a 5% phenyl/95% methylpolysiloxane column. A simple liquid extraction procedure with ethyl acetate was used to isolate the two compounds and the same were separated and analyzed by GC/MS without derivatization. A formal validation of the assay demonstrated good accuracy and precision for both tiletamine (98-100.8%; C.V.total < 6.7%) and zolazepam (98.3-103.4; C.V.total < 13.2%). With 500 μl of plasma, the limits of quantification for both tiletamine and zolazepam were found to be 10 ng/ml. Both compounds were stable after three freeze-thaw cycles. The assay was used to analyze plasma samples collected from a pig after intramuscular administration of 10 mg/kg of Telazol®. The plasma concentration-time profile of tiletamine and zolazepam from this representative pig is also provided.
AB - Tiletamine and zolazepam injection (Telazol®) is used in veterinary surgical practice to induce short-term anesthesia and also to immobilize wild animals. The present work describes a sensitive method to measure tiletamine and zolazepam concentrations in plasma by means of GC/EI-MS on a 5% phenyl/95% methylpolysiloxane column. A simple liquid extraction procedure with ethyl acetate was used to isolate the two compounds and the same were separated and analyzed by GC/MS without derivatization. A formal validation of the assay demonstrated good accuracy and precision for both tiletamine (98-100.8%; C.V.total < 6.7%) and zolazepam (98.3-103.4; C.V.total < 13.2%). With 500 μl of plasma, the limits of quantification for both tiletamine and zolazepam were found to be 10 ng/ml. Both compounds were stable after three freeze-thaw cycles. The assay was used to analyze plasma samples collected from a pig after intramuscular administration of 10 mg/kg of Telazol®. The plasma concentration-time profile of tiletamine and zolazepam from this representative pig is also provided.
KW - GC/MS
KW - Pharmacokinetics
KW - Pig
KW - Tiletamine
KW - Zolazepam
UR - http://www.scopus.com/inward/record.url?scp=33748758948&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33748758948&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2006.05.014
DO - 10.1016/j.jchromb.2006.05.014
M3 - Article
C2 - 16769259
AN - SCOPUS:33748758948
VL - 842
SP - 131
EP - 135
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
SN - 1570-0232
IS - 2 SPEC. ISS.
ER -