Determination of constituents of Telazol®-tiletamine and zolazepam by a gas chromatography/mass spectrometry-based method

Atul Kumar, Henry J. Mann, Rory P Remmel

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Tiletamine and zolazepam injection (Telazol®) is used in veterinary surgical practice to induce short-term anesthesia and also to immobilize wild animals. The present work describes a sensitive method to measure tiletamine and zolazepam concentrations in plasma by means of GC/EI-MS on a 5% phenyl/95% methylpolysiloxane column. A simple liquid extraction procedure with ethyl acetate was used to isolate the two compounds and the same were separated and analyzed by GC/MS without derivatization. A formal validation of the assay demonstrated good accuracy and precision for both tiletamine (98-100.8%; C.V.total < 6.7%) and zolazepam (98.3-103.4; C.V.total < 13.2%). With 500 μl of plasma, the limits of quantification for both tiletamine and zolazepam were found to be 10 ng/ml. Both compounds were stable after three freeze-thaw cycles. The assay was used to analyze plasma samples collected from a pig after intramuscular administration of 10 mg/kg of Telazol®. The plasma concentration-time profile of tiletamine and zolazepam from this representative pig is also provided.

Original languageEnglish (US)
Pages (from-to)131-135
Number of pages5
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume842
Issue number2 SPEC. ISS.
DOIs
StatePublished - Oct 2 2006

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Gas chromatography
Gas Chromatography-Mass Spectrometry
Mass spectrometry
Plasmas
Assays
Zolazepam
Tiletamine
Swine
Wild Animals
zolazepam drug combination tiletamine
Animals
Anesthesia
Injections
Liquids

Keywords

  • GC/MS
  • Pharmacokinetics
  • Pig
  • Tiletamine
  • Zolazepam

Cite this

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title = "Determination of constituents of Telazol{\circledR}-tiletamine and zolazepam by a gas chromatography/mass spectrometry-based method",
abstract = "Tiletamine and zolazepam injection (Telazol{\circledR}) is used in veterinary surgical practice to induce short-term anesthesia and also to immobilize wild animals. The present work describes a sensitive method to measure tiletamine and zolazepam concentrations in plasma by means of GC/EI-MS on a 5{\%} phenyl/95{\%} methylpolysiloxane column. A simple liquid extraction procedure with ethyl acetate was used to isolate the two compounds and the same were separated and analyzed by GC/MS without derivatization. A formal validation of the assay demonstrated good accuracy and precision for both tiletamine (98-100.8{\%}; C.V.total < 6.7{\%}) and zolazepam (98.3-103.4; C.V.total < 13.2{\%}). With 500 μl of plasma, the limits of quantification for both tiletamine and zolazepam were found to be 10 ng/ml. Both compounds were stable after three freeze-thaw cycles. The assay was used to analyze plasma samples collected from a pig after intramuscular administration of 10 mg/kg of Telazol{\circledR}. The plasma concentration-time profile of tiletamine and zolazepam from this representative pig is also provided.",
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author = "Atul Kumar and Mann, {Henry J.} and Remmel, {Rory P}",
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T1 - Determination of constituents of Telazol®-tiletamine and zolazepam by a gas chromatography/mass spectrometry-based method

AU - Kumar, Atul

AU - Mann, Henry J.

AU - Remmel, Rory P

PY - 2006/10/2

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N2 - Tiletamine and zolazepam injection (Telazol®) is used in veterinary surgical practice to induce short-term anesthesia and also to immobilize wild animals. The present work describes a sensitive method to measure tiletamine and zolazepam concentrations in plasma by means of GC/EI-MS on a 5% phenyl/95% methylpolysiloxane column. A simple liquid extraction procedure with ethyl acetate was used to isolate the two compounds and the same were separated and analyzed by GC/MS without derivatization. A formal validation of the assay demonstrated good accuracy and precision for both tiletamine (98-100.8%; C.V.total < 6.7%) and zolazepam (98.3-103.4; C.V.total < 13.2%). With 500 μl of plasma, the limits of quantification for both tiletamine and zolazepam were found to be 10 ng/ml. Both compounds were stable after three freeze-thaw cycles. The assay was used to analyze plasma samples collected from a pig after intramuscular administration of 10 mg/kg of Telazol®. The plasma concentration-time profile of tiletamine and zolazepam from this representative pig is also provided.

AB - Tiletamine and zolazepam injection (Telazol®) is used in veterinary surgical practice to induce short-term anesthesia and also to immobilize wild animals. The present work describes a sensitive method to measure tiletamine and zolazepam concentrations in plasma by means of GC/EI-MS on a 5% phenyl/95% methylpolysiloxane column. A simple liquid extraction procedure with ethyl acetate was used to isolate the two compounds and the same were separated and analyzed by GC/MS without derivatization. A formal validation of the assay demonstrated good accuracy and precision for both tiletamine (98-100.8%; C.V.total < 6.7%) and zolazepam (98.3-103.4; C.V.total < 13.2%). With 500 μl of plasma, the limits of quantification for both tiletamine and zolazepam were found to be 10 ng/ml. Both compounds were stable after three freeze-thaw cycles. The assay was used to analyze plasma samples collected from a pig after intramuscular administration of 10 mg/kg of Telazol®. The plasma concentration-time profile of tiletamine and zolazepam from this representative pig is also provided.

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KW - Tiletamine

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