Determination of chlorpromazine and its major metabolites by gas chromatography/mass spectrometry: Application to biological fluids

L. D. Gruenke, J. Cymerman Craig, F. D. Klein, T. ‐L Nguyen, Barbara A. Hitzemann, J. W. Holaday, H. H. Loh, L. Braff, Ames Fischer, I. D. Glick, F. Hartmann, D. M. Bissell

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Abstract

A method for the quantitative determination of chlorpromazine and five of its major metabolites in a single sample of biological fluid in the ng/ml range has been developed utilizing gas chromatography/mass spectrometry with selected ion recording. The assay is highly specific and quantification is accomplished by an inverse stable isotope dilution technique, using deuterium‐labeled variants of the compounds as internal standards. In this way the concentrations of chlorpromazine and five of its major metabolites (the sulfoxide, the N‐oxide, the monodemethylated, the didemethylated, and the 7‐hydroxylated compounds) can be determined in biological fluids. Levels in humans have been measured both in plasma and in red blood cells and are compared to those found in related in vitro studies.

Original languageEnglish (US)
Pages (from-to)707-713
Number of pages7
JournalBiological Mass Spectrometry
Volume12
Issue number12
DOIs
StatePublished - Dec 1985

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    Gruenke, L. D., Craig, J. C., Klein, F. D., Nguyen, T. L., Hitzemann, B. A., Holaday, J. W., Loh, H. H., Braff, L., Fischer, A., Glick, I. D., Hartmann, F., & Bissell, D. M. (1985). Determination of chlorpromazine and its major metabolites by gas chromatography/mass spectrometry: Application to biological fluids. Biological Mass Spectrometry, 12(12), 707-713. https://doi.org/10.1002/bms.1200121207