Determination of cefmenoxime in human serum by ion-pair reverse-phase high performance liquid chromatography

I. L. Smith; D. J. Swanson; L. S. Welage; C. DeAngelis; S. A. Boudinot; J. J. Schentag

doi:10.1080/00032718508069101

Determination of cefmenoxime in human serum by ion-pair reverse-phase high performance liquid chromatography

I. L. Smith, D. J. Swanson, L. S. Welage, C. DeAngelis, S. A. Boudinot, J. J. Schentag

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Abstract

A highly reproducible ion-pair reverse-phase high performance liquid chromatographic assay for cefmenoxime in human serum has been developed. A simple sample cleanup procedure is employed. Cefoxitin is the internal standard and separation is achieved using a C-18 μ-Bondapak column. The mobile phase consists of 20% acetonitrile and 80% 0.05 M ammonium acetate buffer containing 0.005 M tetrabutylammonium hydrogen sulphate as the ion-pair agent. Samples are quantitated by W detector at 254 nm and 0.02 aufs with an assay sensitivity of 0.625 pg/ml. The method has been successfully applied in a clinical setting.

Original language	English (US)
Pages (from-to)	1077-1085
Number of pages	9
Journal	Analytical Letters
Volume	18
Issue number	9
DOIs	https://doi.org/10.1080/00032718508069101
State	Published - Jan 1985

Keywords

Cefmenoxime Determination by High Performance Liquid Chromatography
Cephalosporin
Serum Levels
colorimetric assay

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10.1080/00032718508069101

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title = "Determination of cefmenoxime in human serum by ion-pair reverse-phase high performance liquid chromatography",

abstract = "A highly reproducible ion-pair reverse-phase high performance liquid chromatographic assay for cefmenoxime in human serum has been developed. A simple sample cleanup procedure is employed. Cefoxitin is the internal standard and separation is achieved using a C-18 μ-Bondapak column. The mobile phase consists of 20% acetonitrile and 80% 0.05 M ammonium acetate buffer containing 0.005 M tetrabutylammonium hydrogen sulphate as the ion-pair agent. Samples are quantitated by W detector at 254 nm and 0.02 aufs with an assay sensitivity of 0.625 pg/ml. The method has been successfully applied in a clinical setting.",

keywords = "Cefmenoxime Determination by High Performance Liquid Chromatography, Cephalosporin, Serum Levels, colorimetric assay",

author = "Smith, {I. L.} and Swanson, {D. J.} and Welage, {L. S.} and C. DeAngelis and Boudinot, {S. A.} and Schentag, {J. J.}",

year = "1985",

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T1 - Determination of cefmenoxime in human serum by ion-pair reverse-phase high performance liquid chromatography

AU - Smith, I. L.

AU - Swanson, D. J.

AU - Welage, L. S.

AU - DeAngelis, C.

AU - Boudinot, S. A.

AU - Schentag, J. J.

PY - 1985/1

Y1 - 1985/1

N2 - A highly reproducible ion-pair reverse-phase high performance liquid chromatographic assay for cefmenoxime in human serum has been developed. A simple sample cleanup procedure is employed. Cefoxitin is the internal standard and separation is achieved using a C-18 μ-Bondapak column. The mobile phase consists of 20% acetonitrile and 80% 0.05 M ammonium acetate buffer containing 0.005 M tetrabutylammonium hydrogen sulphate as the ion-pair agent. Samples are quantitated by W detector at 254 nm and 0.02 aufs with an assay sensitivity of 0.625 pg/ml. The method has been successfully applied in a clinical setting.

AB - A highly reproducible ion-pair reverse-phase high performance liquid chromatographic assay for cefmenoxime in human serum has been developed. A simple sample cleanup procedure is employed. Cefoxitin is the internal standard and separation is achieved using a C-18 μ-Bondapak column. The mobile phase consists of 20% acetonitrile and 80% 0.05 M ammonium acetate buffer containing 0.005 M tetrabutylammonium hydrogen sulphate as the ion-pair agent. Samples are quantitated by W detector at 254 nm and 0.02 aufs with an assay sensitivity of 0.625 pg/ml. The method has been successfully applied in a clinical setting.

KW - Cefmenoxime Determination by High Performance Liquid Chromatography

KW - Cephalosporin

KW - Serum Levels

KW - colorimetric assay

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Determination of cefmenoxime in human serum by ion-pair reverse-phase high performance liquid chromatography

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