Determination of cefmenoxime in human serum by ion-pair reverse-phase high performance liquid chromatography

I. L. Smith, D. J. Swanson, L. S. Welage, C. DeAngelis, S. A. Boudinot, J. J. Schentag

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

A highly reproducible ion-pair reverse-phase high performance liquid chromatographic assay for cefmenoxime in human serum has been developed. A simple sample cleanup procedure is employed. Cefoxitin is the internal standard and separation is achieved using a C-18 μ-Bondapak column. The mobile phase consists of 20% acetonitrile and 80% 0.05 M ammonium acetate buffer containing 0.005 M tetrabutylammonium hydrogen sulphate as the ion-pair agent. Samples are quantitated by W detector at 254 nm and 0.02 aufs with an assay sensitivity of 0.625 pg/ml. The method has been successfully applied in a clinical setting.

Original languageEnglish (US)
Pages (from-to)1077-1085
Number of pages9
JournalAnalytical Letters
Volume18
Issue number9
DOIs
StatePublished - Jan 1985

Keywords

  • Cefmenoxime Determination by High Performance Liquid Chromatography
  • Cephalosporin
  • Serum Levels
  • colorimetric assay

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