Abstract
A highly reproducible ion-pair reverse-phase high performance liquid chromatographic assay for cefmenoxime in human serum has been developed. A simple sample cleanup procedure is employed. Cefoxitin is the internal standard and separation is achieved using a C-18 μ-Bondapak column. The mobile phase consists of 20% acetonitrile and 80% 0.05 M ammonium acetate buffer containing 0.005 M tetrabutylammonium hydrogen sulphate as the ion-pair agent. Samples are quantitated by W detector at 254 nm and 0.02 aufs with an assay sensitivity of 0.625 pg/ml. The method has been successfully applied in a clinical setting.
Original language | English (US) |
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Pages (from-to) | 1077-1085 |
Number of pages | 9 |
Journal | Analytical Letters |
Volume | 18 |
Issue number | 9 |
DOIs | |
State | Published - Jan 1985 |
Keywords
- Cefmenoxime Determination by High Performance Liquid Chromatography
- Cephalosporin
- Serum Levels
- colorimetric assay