Determination of bile acid pool size in man: a simplified method with advantages of increased precision, shortened analysis time, and decreased isotope exposure

W. C. Duane, R. D. Adler, L. J. Bennion, R. L. Ginsberg

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

A simplified isotope dilution method for measurement of the bile acid pool size in normal subjects is described and compared with the traditional method of Lindstedt. Advantages of this simplified method include a four to eightfold reduction of isotope dose, facilitation of analytical procedures and a reduction in the required number of duodenal intubations. In 15 human subjects who had two separate estimates of pool size by this method, precision averaged 2.6%. In 16 comparisons, pool size measured by this method averaged 13.7% higher than simultaneous estimates by the Lindstedt method. Factors affecting accuracy (as opposed to precision) in both methods are discussed.

Original languageEnglish (US)
Pages (from-to)155-158
Number of pages4
JournalJournal of lipid research
Volume16
Issue number2
StatePublished - 1975

Fingerprint

Dive into the research topics of 'Determination of bile acid pool size in man: a simplified method with advantages of increased precision, shortened analysis time, and decreased isotope exposure'. Together they form a unique fingerprint.

Cite this