A simplified isotope dilution method for measurement of the bile acid pool size in normal subjects is described and compared with the traditional method of Lindstedt. Advantages of this simplified method include a four to eightfold reduction of isotope dose, facilitation of analytical procedures and a reduction in the required number of duodenal intubations. In 15 human subjects who had two separate estimates of pool size by this method, precision averaged 2.6%. In 16 comparisons, pool size measured by this method averaged 13.7% higher than simultaneous estimates by the Lindstedt method. Factors affecting accuracy (as opposed to precision) in both methods are discussed.
|Original language||English (US)|
|Number of pages||4|
|Journal||Journal of lipid research|
|State||Published - 1975|