A rapid and sensitive method was developed for the quantitative determination of α-tocopherol in tissues and plasma of rats and mice. Tissue and plasma were extracted in acetone and chromatographed on a reverse-phase C18 column with 2% water in methanol. Fluorescence and ultraviolet detection were used for tissue and plasma α-tocopherol levels, respectively. Extraction of tissues and plasma was found to be more complete in acetone than in other solvent systems analyzed. The average recovery of α-tocopherol added to tissue samples was 97%. As little as 0.1 g of tissue or 0.1 ml plasma can be accurately used for analysis. The method is sensitive to 0.05 μg α-tocopherol/g tissue.
Bibliographical noteFunding Information:
This work was supported in part by NIEH ES02325 02 and by the Minnesota Agricultural Experiment Station Grant No. MN 18-085.