A method based on liquid chromatography (LC) combined with electrospray ionization tandem mass spectrometry for the analysis of the frequency of formation of 8-hydroxydeoxyguanosine (8OHdGuo), a biomarker of oxidative damage to DNA, has been developed. This analytical technique has the advantage over gas chromatography combined with mass spectrometry of not requiring analyte derivatization, and over LC combined with ultraviolet and electrochemical detection of being chemospecific. The method was evaluated by determining increased frequency of formation of 8OHdGuo in male Sprague- Dawley cats given a single intraperitoneal dose of Adriamycin® compared to control rats. Detection of one oxidized deoxyguanosine per 5 x 105 deoxyguanosines was readily achieved.
|Original language||English (US)|
|Number of pages||3|
|Journal||Rapid Communications in Mass Spectrometry|
|State||Published - Dec 1 1996|