Determination of 8-hydroxydeoxyguanosine in biological tissue by liquid chromatography/electrospray ionization-mass spectrometry/mass spectrometry

Jose Serrano; Carlos M. Palmeira; Kendall B Wallace; Douglas W. Kuehl

doi:10.1002/(SICI)1097-0231(199611)10:14<1789::AID-RCM752>3.0.CO;2-6

Determination of 8-hydroxydeoxyguanosine in biological tissue by liquid chromatography/electrospray ionization-mass spectrometry/mass spectrometry

Jose Serrano, Carlos M. Palmeira, Kendall B Wallace, Douglas W. Kuehl

Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

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Abstract

A method based on liquid chromatography (LC) combined with electrospray ionization tandem mass spectrometry for the analysis of the frequency of formation of 8-hydroxydeoxyguanosine (8OHdGuo), a biomarker of oxidative damage to DNA, has been developed. This analytical technique has the advantage over gas chromatography combined with mass spectrometry of not requiring analyte derivatization, and over LC combined with ultraviolet and electrochemical detection of being chemospecific. The method was evaluated by determining increased frequency of formation of 8OHdGuo in male Sprague- Dawley cats given a single intraperitoneal dose of Adriamycin® compared to control rats. Detection of one oxidized deoxyguanosine per 5 x 10⁵ deoxyguanosines was readily achieved.

Original language	English (US)
Pages (from-to)	1789-1791
Number of pages	3
Journal	Rapid Communications in Mass Spectrometry
Volume	10
Issue number	14
DOIs	https://doi.org/10.1002/(SICI)1097-0231(199611)10:14<1789::AID-RCM752>3.0.CO;2-6
State	Published - Dec 1 1996

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10.1002/(SICI)1097-0231(199611)10:14<1789::AID-RCM752>3.0.CO;2-6

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abstract = "A method based on liquid chromatography (LC) combined with electrospray ionization tandem mass spectrometry for the analysis of the frequency of formation of 8-hydroxydeoxyguanosine (8OHdGuo), a biomarker of oxidative damage to DNA, has been developed. This analytical technique has the advantage over gas chromatography combined with mass spectrometry of not requiring analyte derivatization, and over LC combined with ultraviolet and electrochemical detection of being chemospecific. The method was evaluated by determining increased frequency of formation of 8OHdGuo in male Sprague- Dawley cats given a single intraperitoneal dose of Adriamycin{\textregistered} compared to control rats. Detection of one oxidized deoxyguanosine per 5 x 105 deoxyguanosines was readily achieved.",

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AU - Wallace, Kendall B

AU - Kuehl, Douglas W.

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Determination of 8-hydroxydeoxyguanosine in biological tissue by liquid chromatography/electrospray ionization-mass spectrometry/mass spectrometry

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