Cohesin proteins help maintain the physical associations between sister chromatids that arise in S-phase and are removed in anaphase. Recent studies found that cohesins also localize to the centrosomes, the organelles that organize the mitotic bipolar spindle. We find that the cohesin protein Rad21 localizes to centrosomes in a manner that is dependent upon known regulators of sister chromatid cohesion as well as regulators of centrosome function. These data suggest that Rad21 functions at the centrosome and that the regulators of Rad21 coordinate the centrosome and chromosomal functions of cohesin.
Bibliographical noteFunding Information:
We thank R. Kuriyama for ongoing discussions throughout the project and for critical reading of the manuscript. We thank K. Sullivan, S. Doxsey, S. Jensen, J. Peters and H. Yu for reagents. L.A.D.M. was partially funded by CONACyT, J.F.G.A. by BFU2008-03579/BMC, and D.J.C. by a seed grant from the University of Minnesota Academic Health Center.