Abstract
Modified Drosophila heat shock genes were introduced into the germ line by P element transformation. The genes were altered such that several factors could be tested for their influence upon chromosome puffing. Deletion of promoter sequences upstream of position -73 of an hsp7O-lacZ hybrid gene was sufficient to abolish puffing. Analysis of progressive 5′ deletions defines a 16 bp interval that contains sequences required for both heat-induced puffing and gene expression. An internal deletion of the hsp70-lacZ gene that reduces the transcript size from 9 kb to 0.8 kb results in a dramatic reduction in puff size. The chromosomal insertion sites of 26 variant hsp70 or hsp26 genes fall to influence puffing greatly with one marked exception. This transformant possesses an insert that falls to puff and exhibits a tissue-restricted pattern of expression. These results indicate that variation in either promoter strength or transcript length have profound effects on puffing.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 805-817 |
| Number of pages | 13 |
| Journal | Cell |
| Volume | 40 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 1985 |
Bibliographical note
Funding Information:We thank Janis Werner for invaluable assistance with embryo microinjections. We also thank J. Posakony and A. Chovnick for providing fly stocks used in this work, G. Rubin and A. Spradling for providing transformation vectors prior to publication, and M. Wolfner and M. Goldberg for helpful comments on the manuscript. This work was supported by National Institutes of Health grant GM25232 to J. L. and National Research Service Award GM07232 to J. S. and to R. G.