Detection of transgenic maize Cry1Ab protein subjected to ruminal digestion

J. L. Jacobs, F. Diez-Gonzalez, M. D. Stern, R. L. Phillips

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1 Scopus citations

Abstract

This research determined the extent of ruminal degradation of a transgenic protein (Cry1Ab) expressed in kernels of maize (Zea mays) cultivar DKC53-32. Cracked kernels of transgenic maize (Bt+) were subjected to rumen digestion using in situ experiments with two ruminally cannulated Holstein cows. A non-transgenic maize cultivar (Bt-) was used as a control for crude protein (CP) degradation. CP and Cry1A concentrations from dacron polyester bags inserted in the rumen were determined for 48 h. The Bt- cultivar had greater CP content (P<0.05) than the Bt+ maize, but no difference (P>0.05) was found in ruminal degradable protein and the CP rate of degradation. The Cry1Ab protein was detected throughout ruminal digestion. The amount of Cry1Ab protein remaining after 16 and 24 h of ruminal degradation was 85 and 60%, respectively. These results indicated that a significant fraction of Cry1Ab protein was not digested in the rumen. This is the first report of the rumen degradation rate of a transgenic protein.

Original languageEnglish (US)
Pages (from-to)655-664
Number of pages10
JournalJournal of Animal and Feed Sciences
Volume14
Issue number4
DOIs
StatePublished - 2005

Keywords

  • Bt maize
  • Cattle
  • Cry1Ab protein
  • Genetically modified
  • Rumen digestion
  • Zea mays

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