Rotavirus (RV) is an important cause of gastrointestinal disease in animals and humans. In this study, we developed an RT-PCR to detect RV group B (RVB) and characterized the VP7 (G) gene segment detected in porcine samples. One hundred seventy three samples were tested for RV group A (RVA), RVB, and C (RVC) by RT-PCR and examined for RV-like lesion using histopathology. A majority (86.4%) of the samples had mixed RV infections and co-infections of RVA/RVB/RVC were detected at a higher rate (24.3%) than previously reported. RVB was identified in 46.8% of the 173 samples. An adapted VP7 classification was developed using previously published (n=57) and newly sequenced (n=68) RVB strains, resulting in 20 G genotypes based on an 80% nucleotide identity cutoff value. Our results revealed a broad genetic diversity of porcine RVB strains, suggesting RVB has been the cause of common/pre-existing, yet undiagnosed, disease in pigs.
Bibliographical noteFunding Information:
We thank Mary Thurn and Dr. Yin Jiang at the University of Minnesota Veterinary Diagnostic Laboratory (UMVDL) for their technical assistance. Research was supported by the UMVDL J.M was supported by an FWO (‘Fonds voor Wetenschappelijk Onderzoek’) post-doctoral fellowship.
- Gastrointestinal disease
- Phylogenetic analysis
- Porcine enteric disease
- Rotavirus group A
- Rotavirus group B
- Rotavirus group C