Detection of phospholipid peroxides in biological samples

Frederik J.G.M. van Kuijk, Edward A. Dratz

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


Peroxidation of membrane lipids has been hypothesized to play a key role in various types of tissue degeneration and pathology. Lipid peroxides are formed when oxygen reacts with an unsaturated fatty acid chain. Virtually all of the unsaturated fatty acids in biological systems are bound by ester linkages in phospholipids or triglycerides. Phospholipid and triglyceride peroxides are primary products of lipid peroxidation and have rarely been measured. Most of the commonly used methods for detection of lipid peroxidation are based on detection of malondialdehyde or other chemical species that are derived from oxidized fatty acids. This review presents an overview of recently developed methods aimed at identifying and measuring oxidized phospholipids and triglycerides which are direct evidence of the occurrence of lipid peroxidation in vivo.

Original languageEnglish (US)
Pages (from-to)349-354
Number of pages6
JournalFree Radical Biology and Medicine
Issue number5
StatePublished - 1987
Externally publishedYes

Bibliographical note

Funding Information:
Acknowledgements--This work was supported by grants from The Netherlands Organization for the Advancement of Pure Research (ZWO) (to FJGMvK) and the National Institutes of Health (to EAD).


  • Colorimetric assay
  • Enzymatic assay
  • Gas chromatography-mass spectrometry
  • High performance liquid chromatography
  • Lipid autoxidation
  • Lipid peroxidation
  • Phospholipid peroxides
  • Ultraviolet absorption spectra


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