Detection of phase separation in fluid phosphatidylserine/phosphatidylcholine mixtures

A. K. Hinderliter, J. Huang, G. W. Feigenson

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The nonideal mixing of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoserine, (16:0, 18:1)PS, and 1,2-didodecenoyl-sn-glycero-3-phosphocholine, (12:1, 12:1)PC, in fluid lamellar model membranes was studied by measuring binding of aqueous Ca2+ ions and by x-ray diffraction. A region of two-phase coexistence was found by invariance of the aqueous concentration and by the appearance of two sets of lamellar spacings. The phases were identified as fluid from the diffuse x-ray diffraction in the wide-angle region. The width of the two-phase coexistence region was greater at higher ionic strength. In 800 mM KCl, the phase boundaries were at PS mole fraction 0.5 and 0.8. In 100 mM KCl, the phase boundaries were at PS mole fraction 0.52 and 0.62. Monte Carlo simulations of the lateral distributions of these PS/PC mixtures show pronounced clustering of the lipids.

Original languageEnglish (US)
Pages (from-to)1906-1911
Number of pages6
JournalBiophysical journal
Issue number5
StatePublished - 1994

Bibliographical note

Funding Information:
We wish to thank Joe Navaie for help with the x-ray diffraction measure- ments at CHESS, Andy Dibble for help with the 31PNMR experiments, and Marian Szebenyi for help with the densitometer and for providing the plot- ting programs. This work was supported by National Institutes of Health grant HL-18255. A.K.H. was supported in part by NIH grant 1-T32-GM08267.


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