Detection of PERK Signaling in the Central Nervous System

Zhixin Lei, Sarrabeth M Stone, Wensheng Lin

Research output: Chapter in Book/Report/Conference proceedingChapter


In response to endoplasmic reticulum (ER) stress, activation of pancreatic ER kinase (PERK) signaling adapts cells to stressful conditions by phosphorylating eukaryotic translation initiation factor 2α (eIF2α). Phosphorylation of eIF2α inhibits global protein translation but stimulates the expression of numerous stress-responsive genes by inducing the transcription factor ATF4. A large number of studies have shown that activation of PERK signaling has beneficial or detrimental effects in various diseases of the central nervous system (CNS), including neurodegenerative diseases, myelin disorders, CNS injuries, among others. This chapter is devoted to describing the practical methods for the detection of PERK signaling in CNS diseases.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages13
StatePublished - 2022

Publication series

NameMethods in molecular biology (Clifton, N.J.)
PublisherHumana Press
ISSN (Print)1064-3745

Bibliographical note

Funding Information:
This study was supported by grants from the National Institutes of Health (R01NS094151 and R01NS105689) to Wensheng Lin.

Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.


  • ATF4
  • CHOP
  • GADD34
  • Immunofluorescence
  • Neuron
  • Oligodendrocyte
  • PERK
  • Phosphorylated eIF2α
  • Real-time PCR
  • Western blot

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural


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