Detection of O6-Methvldeoxveuanosine in Human Placental DNA

Peter G. Foiles, Lisa M. Miglietta, Shobha A. Akerkar, Richard B. Everson, Stephen S. Hecht

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Abstract

A monoclonal antibody specific for O6-methyldeoxyguanosine (O6-MedGuo) was developed. When used in a competitive enzyme-linked immunosorbent assay, 50% inhibition of binding was achieved with 0.51 pmol O6-MedGuo. When the competitive enzyme-linked immunosorbent assay was coupled with high-performance liquid chromatography, 2 mg of DNA could be analyzed giving a lower limit of detection of 0.5 μmol O6-MedGuo/mol deoxyguanosine. This assay was used to test for O6-MedGuo in DNA from placentas of smoking and nonsmoking women. Two of 10 DNA samples from smoking women and three of 10 from nonsmoking women had detectable concentrations of O6-MedGuo. Concentrations ranged from 0.6 to 1.6 μmolO6-MedGuo/mol deoxyguano-sine. Activity of O6-alkylguanine DNA alkyltransferase was also measured. There was no apparent relationship between O6-alkylguanine DNA alkyltransferase activity and O6-MedGuo concentrations in the 20 subjects, nor did mean O6-alkylguanine DNA alkyltransferase activity differ between the two groups. Although no apparent relationship between smoking history and O6-MedGuo concentration was found in this preliminary study, this is the first report of a structurally identified DNA adduct in human placenta.

Original languageEnglish (US)
Pages (from-to)4184-4188
Number of pages5
JournalCancer Research
Volume48
Issue number15
StatePublished - Jan 1 1988

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    Foiles, P. G., Miglietta, L. M., Akerkar, S. A., Everson, R. B., & Hecht, S. S. (1988). Detection of O6-Methvldeoxveuanosine in Human Placental DNA. Cancer Research, 48(15), 4184-4188.