Detection of insulin-like growth factor binding proteins (IGFBPs) by ligand blotting in breast cancer tissues

Sean E. McGuire, Susan G. Hilsenbeck, JoséA Figueroa, James G. Jackson, Douglas Yee

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Eighty breast cancer specimens were examined for insulin-like growth factor binding protein (IGFBP) expression by ligand blotting. Five distinct IGFBP species were found: a doublet at 48 and 44 kDa was IGFBP-3, the 34-kDa band was IGFBP-2, and a band at 24 kDa was IGFBP-4. A 32-kDa band was compatible with the migration position reported for IGFBP-5. IGFBP-3 was inversely correlated with ER expression, while IGFBP-4 was positively correlated with both ER and PgR. IGFBP-4 was also inversely correlated with S-phase fraction. Thus, IGFBP expression correlates with other parameters of breast cancer biology and may play a role in regulating tumor growth.

Original languageEnglish (US)
Pages (from-to)25-32
Number of pages8
JournalCancer Letters
Volume77
Issue number1
DOIs
StatePublished - Feb 28 1994

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Insulin-Like Growth Factor Binding Protein 4
Insulin-Like Growth Factor Binding Proteins
Insulin-Like Growth Factor Binding Protein 3
Breast Neoplasms
Ligands
Insulin-Like Growth Factor Binding Protein 5
Insulin-Like Growth Factor Binding Protein 2
S Phase
Growth
Neoplasms

Keywords

  • Breast cancer
  • Estrogen receptor
  • Insulin-like growth factor binding proteins
  • Progesterone receptor
  • S-phase fraction

Cite this

Detection of insulin-like growth factor binding proteins (IGFBPs) by ligand blotting in breast cancer tissues. / McGuire, Sean E.; Hilsenbeck, Susan G.; Figueroa, JoséA; Jackson, James G.; Yee, Douglas.

In: Cancer Letters, Vol. 77, No. 1, 28.02.1994, p. 25-32.

Research output: Contribution to journalArticle

McGuire, Sean E. ; Hilsenbeck, Susan G. ; Figueroa, JoséA ; Jackson, James G. ; Yee, Douglas. / Detection of insulin-like growth factor binding proteins (IGFBPs) by ligand blotting in breast cancer tissues. In: Cancer Letters. 1994 ; Vol. 77, No. 1. pp. 25-32.
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