Detection of DNA methylation by whole-genome bisulfite sequencing

Qing Li, Peter J. Hermanson, Nathan M. Springer

Research output: Chapter in Book/Report/Conference proceedingChapter

12 Scopus citations

Abstract

DNA methylation plays an important role in the regulation of the expression of transposons and genes. Various methods have been developed to assay DNA methylation levels. Bisulfite sequencing is considered to be the “gold standard” for single-base resolution measurement of DNA methylation levels. Coupled with next-generation sequencing, whole-genome bisulfite sequencing (WGBS) allows DNA methylation to be evaluated at a genome-wide scale. Here, we described a protocol for WGBS in plant species with large genomes. This protocol has been successfully applied to assay genome-wide DNA methylation levels in maize and barley. This protocol has also been successfully coupled with sequence capture technology to assay DNA methylation levels in a targeted set of genomic regions.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages185-196
Number of pages12
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1676
ISSN (Print)1064-3745

Keywords

  • Bisulfite conversion
  • DNA methylation
  • Sequence capture
  • Sequencing
  • Single-base resolution

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