Detection of DNA methylation by whole-genome bisulfite sequencing

Qing Li, Peter J. Hermanson, Nathan M Springer

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

DNA methylation plays an important role in the regulation of the expression of transposons and genes. Various methods have been developed to assay DNA methylation levels. Bisulfite sequencing is considered to be the “gold standard” for single-base resolution measurement of DNA methylation levels. Coupled with next-generation sequencing, whole-genome bisulfite sequencing (WGBS) allows DNA methylation to be evaluated at a genome-wide scale. Here, we described a protocol for WGBS in plant species with large genomes. This protocol has been successfully applied to assay genome-wide DNA methylation levels in maize and barley. This protocol has also been successfully coupled with sequence capture technology to assay DNA methylation levels in a targeted set of genomic regions.

LanguageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages185-196
Number of pages12
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1676
ISSN (Print)1064-3745

Fingerprint

DNA Methylation
Genome
Gene Expression Regulation
Hordeum
Zea mays
hydrogen sulfite
Technology

Keywords

  • Bisulfite conversion
  • DNA methylation
  • Sequence capture
  • Sequencing
  • Single-base resolution

Cite this

Li, Q., Hermanson, P. J., & Springer, N. M. (2018). Detection of DNA methylation by whole-genome bisulfite sequencing. In Methods in Molecular Biology (pp. 185-196). (Methods in Molecular Biology; Vol. 1676). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7315-6_11

Detection of DNA methylation by whole-genome bisulfite sequencing. / Li, Qing; Hermanson, Peter J.; Springer, Nathan M.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 185-196 (Methods in Molecular Biology; Vol. 1676).

Research output: Chapter in Book/Report/Conference proceedingChapter

Li, Q, Hermanson, PJ & Springer, NM 2018, Detection of DNA methylation by whole-genome bisulfite sequencing. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1676, Humana Press Inc., pp. 185-196. https://doi.org/10.1007/978-1-4939-7315-6_11
Li Q, Hermanson PJ, Springer NM. Detection of DNA methylation by whole-genome bisulfite sequencing. In Methods in Molecular Biology. Humana Press Inc. 2018. p. 185-196. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7315-6_11
Li, Qing ; Hermanson, Peter J. ; Springer, Nathan M. / Detection of DNA methylation by whole-genome bisulfite sequencing. Methods in Molecular Biology. Humana Press Inc., 2018. pp. 185-196 (Methods in Molecular Biology).
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