Detection of CWD prions in urine and saliva of deer by transgenic mouse bioassay

Nicholas J. Haley, Davis M. Seelig, Mark D. Zabel, Glenn C. Telling, Edward A. Hoover

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

Chronic wasting disease (CWD) is a prion disease affecting captive and free-ranging cervids (e.g. deer, elk, and moose). The mechanisms of CWD transmission are poorly understood, though bodily fluids are thought to play an important role. Here we report the presence of infectious prions in the urine and saliva of deer with chronic wasting disease (CWD). Prion infectivity was detected by bioassay of concentrated, dialyzed urine and saliva in transgenic mice expressing the cervid PrP gene (Tg[CerPrP] mice). In addition, PrPCWD was detected in pooled and concentrated urine by protein misfolding cyclic amplification (PMCA). The concentration of abnormal prion protein in bodily fluids was very low, as indicated by: undetectable PrPCWD levels by traditional assays (western blot, ELISA) and prolonged incubation periods and incomplete TSE attack rates in inoculated Tg(CerPrP) mice (373±3days in 2 of 9 urine-inoculated mice and 342±109 days in 8 of 9 saliva-inoculated mice). These findings help extend our understanding of CWD prion shedding and transmission and portend the detection of infectious prions in body fluids in other prion infections.

Original languageEnglish (US)
Article numbere4848
JournalPloS one
Volume4
Issue number3
DOIs
StatePublished - Mar 18 2009

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Chronic Wasting Disease
Peromyscus
chronic wasting disease
Bioassay
Prions
prions
saliva
Saliva
Biological Assay
deer
Transgenic Mice
urine
bioassays
Urine
genetically modified organisms
mice
Deer
Genes
Prion Diseases
Body Fluids

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Detection of CWD prions in urine and saliva of deer by transgenic mouse bioassay. / Haley, Nicholas J.; Seelig, Davis M.; Zabel, Mark D.; Telling, Glenn C.; Hoover, Edward A.

In: PloS one, Vol. 4, No. 3, e4848, 18.03.2009.

Research output: Contribution to journalArticle

Haley, Nicholas J. ; Seelig, Davis M. ; Zabel, Mark D. ; Telling, Glenn C. ; Hoover, Edward A. / Detection of CWD prions in urine and saliva of deer by transgenic mouse bioassay. In: PloS one. 2009 ; Vol. 4, No. 3.
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