Detection of Bartonella henselae DNA in two dogs with pyogranulomatous lymphadenitis

Sofia C. Morales, Edward B. Breitschwerdt, Robert J Washabau, VMD, PhD, Ilze Matise, Ricardo G. Maggi, Ashlee W. Duncan

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Case Description - 1 dog evaluated because of inappetence and lameness of the left hind limb of 1 day's duration and 1 dog evaluated because of inappetence, fever, and lymphadenopathy of 2 weeks' duration. Clinical Findings - Histologic examination of excisional biopsy specimens from lymph nodes revealed pyogranulomatous lymphadenitis in both dogs. Quantitative real-time PCR assays detected Bartonella henselae DNA in blood samples and affected lymph node specimens from both dogs. Antibodies against B henselae were not detected via immunofluorescent antibody testing during active disease in either dog. Treatment and Outcome - 1 dog recovered after 6 weeks of treatment with doxycycline (5 mg/kg [2.3 mg/lb], PO, q 12 h), whereas the other dog recovered after receiving a combination of azithromycin (14.5 mg/kg [6.6 mg/lb], PO, q 24 h for 21 days), doxycycline (17.3 mg/kg [7.9 mg/lb], PO, q 24 h for 4 weeks), and immunosuppressive corticosteroid (prednisone [3 mg/kg {1.4 mg/lb}, PO, q 24 h], tapered by decreasing the daily dose by 25% every 2 weeks) treatment. Clinical Relevance - B henselae is implicated as a possible cause or a cofactor in the development of pyogranulomatous lymphadenitis in dogs. In dogs with pyogranulomatous lymphadenitis, immunofluorescent assays may not detect antibodies against B henselae. Molecular testing, including PCR assay of affected tissues, may provide an alternative diagnostic method for detection of B henselae DNA in pyogranulomatous lymph nodes.

Original languageEnglish (US)
Pages (from-to)681-685
Number of pages5
JournalJournal of the American Veterinary Medical Association
Volume230
Issue number5
DOIs
StatePublished - Mar 1 2007

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Bartonella henselae
lymphadenitis
Lymphadenitis
Dogs
dogs
DNA
lymph nodes
doxycycline
Doxycycline
Lymph Nodes
Antibodies
azithromycin
prednisone
immunosuppressive agents
lymphatic diseases
antibodies
duration
dog diseases
Azithromycin
assays

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Detection of Bartonella henselae DNA in two dogs with pyogranulomatous lymphadenitis. / Morales, Sofia C.; Breitschwerdt, Edward B.; Washabau, VMD, PhD, Robert J; Matise, Ilze; Maggi, Ricardo G.; Duncan, Ashlee W.

In: Journal of the American Veterinary Medical Association, Vol. 230, No. 5, 01.03.2007, p. 681-685.

Research output: Contribution to journalArticle

Morales, Sofia C. ; Breitschwerdt, Edward B. ; Washabau, VMD, PhD, Robert J ; Matise, Ilze ; Maggi, Ricardo G. ; Duncan, Ashlee W. / Detection of Bartonella henselae DNA in two dogs with pyogranulomatous lymphadenitis. In: Journal of the American Veterinary Medical Association. 2007 ; Vol. 230, No. 5. pp. 681-685.
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