Detection of Bartonella henselae and Bartonella clarridgeiae DNA in hepatic specimens from two dogs with hepatic disease

Tracey N. Gillespie, Robert J. Washabau, Michael H. Goldschmidt, John M. Cullen, Allison R. Rogala, Edward B. Breitschwerdt

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

A 4-year-old Basset Hound and a 6-year-old Doberman Pinscher were referred for diagnostic evaluation following documentation of persistently increased hepatic enzyme activities and hepatic dysfunction. Histologic evaluation of hepatic biopsy specimens from the 2 dogs revealed granulomatous hepatitis in the Basset Hound and lymphocytic hepatitis with fibrosis and copper accumulation in the Doberman Pinscher. No etiologic agents were identified histologically. Bartonetta henselae DNA was subsequently amplified from hepatic tissue from the Basset Hound and Bartonella clarridgeiae was amplified from hepatic tissue from the Doberman Pinscher. Amplification was performed with a polymerase chain reaction assay incorporating primers that target a portion of the 16S-23S rRNA intergenic spacer region. Both dogs were treated with azithromycin, in combination with a variety of other medications and herbal treatments, and improved clinically. Identification of Bartonella DNA in these dogs indicates the need for future prospective studies to determine the clinical relevance of Bartonella spp infection in dogs with hepatic disease.

Original languageEnglish (US)
Pages (from-to)47-51+35
JournalJournal of the American Veterinary Medical Association
Volume222
Issue number1
DOIs
StatePublished - Jan 1 2003

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Bartonella clarridgeiae
Bartonella henselae
Bartonella
liver diseases
Doberman Pinscher
hounds
Dogs
liver
dogs
Liver
DNA
hepatitis
Hepatitis
azithromycin
Bartonella Infections
Azithromycin
Intergenic DNA
prospective studies
intergenic DNA
fibrosis

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Detection of Bartonella henselae and Bartonella clarridgeiae DNA in hepatic specimens from two dogs with hepatic disease. / Gillespie, Tracey N.; Washabau, Robert J.; Goldschmidt, Michael H.; Cullen, John M.; Rogala, Allison R.; Breitschwerdt, Edward B.

In: Journal of the American Veterinary Medical Association, Vol. 222, No. 1, 01.01.2003, p. 47-51+35.

Research output: Contribution to journalArticle

Gillespie, Tracey N. ; Washabau, Robert J. ; Goldschmidt, Michael H. ; Cullen, John M. ; Rogala, Allison R. ; Breitschwerdt, Edward B. / Detection of Bartonella henselae and Bartonella clarridgeiae DNA in hepatic specimens from two dogs with hepatic disease. In: Journal of the American Veterinary Medical Association. 2003 ; Vol. 222, No. 1. pp. 47-51+35.
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