Detection of bacterial β-galactosidase activity in individual Saccharomyces cerevisiae cells by flow cytometry

Friedrich Srienc; Judith L. Campbell; James E. Bailey

doi:10.1007/BF00189963

Detection of bacterial β-galactosidase activity in individual Saccharomyces cerevisiae cells by flow cytometry

Friedrich Srienc, Judith L. Campbell, James E. Bailey

Biotechnology Institute

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36 Scopus citations

Abstract

A fluorogenic substrate and trapping reagent have been used to detect β-galactosidase activity expressed in S. cerevisiae from an Escherichia coli lacZ gene segment encoded on a recombinant plasmid. The measurement may be made in less than 1 msec using a flow cytometer, facilitating rapid experimental characterization of plasmid instability.

Original language	English (US)
Pages (from-to)	43-48
Number of pages	6
Journal	Biotechnology Letters
Volume	5
Issue number	1
DOIs	https://doi.org/10.1007/BF00189963
State	Published - Jan 1983

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title = "Detection of bacterial β-galactosidase activity in individual Saccharomyces cerevisiae cells by flow cytometry",

abstract = "A fluorogenic substrate and trapping reagent have been used to detect β-galactosidase activity expressed in S. cerevisiae from an Escherichia coli lacZ gene segment encoded on a recombinant plasmid. The measurement may be made in less than 1 msec using a flow cytometer, facilitating rapid experimental characterization of plasmid instability.",

author = "Friedrich Srienc and Campbell, {Judith L.} and Bailey, {James E.}",

year = "1983",

month = jan,

doi = "10.1007/BF00189963",

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volume = "5",

pages = "43--48",

journal = "Biotechnology Letters",

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AU - Srienc, Friedrich

AU - Campbell, Judith L.

AU - Bailey, James E.

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N2 - A fluorogenic substrate and trapping reagent have been used to detect β-galactosidase activity expressed in S. cerevisiae from an Escherichia coli lacZ gene segment encoded on a recombinant plasmid. The measurement may be made in less than 1 msec using a flow cytometer, facilitating rapid experimental characterization of plasmid instability.

AB - A fluorogenic substrate and trapping reagent have been used to detect β-galactosidase activity expressed in S. cerevisiae from an Escherichia coli lacZ gene segment encoded on a recombinant plasmid. The measurement may be made in less than 1 msec using a flow cytometer, facilitating rapid experimental characterization of plasmid instability.

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IS - 1

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Detection of bacterial β-galactosidase activity in individual Saccharomyces cerevisiae cells by flow cytometry

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