Detection of avian pneumovirus in wild Canada geese (Branta canadensis) and blue-winged teal (Anas discors)

R. S. Bennett, B. McComb, H. J. Shin, M. K. Njenga, K. V. Nagaraja, D. A. Halvorson

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Choanal cleft swab samples from 770 wild Canada geese (Branta canadensis) and 358 blue-winged teal (Anas discors), captured for relocation or banding, were examined for the presence of avian pneumovirus (APV) RNA by reverse transcription (RT)-polymerase chain reaction (PCR) and for virus isolation. The swab samples were pooled into groups of 5 or 10. Sixty eight of 102 (66.7%) pooled goose samples were RT-PCR positive for APV RNA. Thirteen of 52 (25.0%) pooled blue-winged teal samples were RT-PCR positive for APV RNA. APV RNA-positive samples were inoculated onto chick embryo fibroblasts (CEF) and QT-35 cells. Infectious APV was isolated from five Canada goose pooled samples in CEF and from one Canada goose pool in QT-35 cells but not from blue-winged teal.

Original languageEnglish (US)
Pages (from-to)1025-1029
Number of pages5
JournalAvian diseases
Volume46
Issue number4
DOIs
StatePublished - Jan 1 2002

Keywords

  • Anas discors
  • Avian pneumovirus
  • Blue-winged teal
  • Branta canadensis
  • Canada goose

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