Detection of Antiplatelet Antibody With a Platelet Immunofluorescence Assay

Annemarie T. Kristensen, Douglas J. Weiss, Jeffrey S Klausner, Judy Laber, Douglas J. Christie

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

An indirect platelet immunofluorescence assay (PIFA) was developed for detection of circulating antiplatelet antibody in dogs with suspected immune‐mediated thrombocytopenia (ITP). The PIFA was performed on 10 healthy dogs with normal platelet counts; 76 thrombocytopenic dogs, 20 of which were suspected of having ITP; and 18 dogs with other diseases and normal platelet counts. All normal dogs had negative test results. Fourteen (70%) of 20 dogs suspected of having ITP had positive test results. Fifteen of the remaining 56 thrombocytopenic dogs had positive test results, 9 had cancer and 6 had other immune‐mediated diseases including systemic lupus erythematosus (SLE). In this study, the PIFA assay seemed to be more sensitive (70%) than the megakaryocyte immunofluorescence assay (41 %) in the diagnosis of ITP. Of the 9 PIFA‐positive dogs with neoplasia, 6 had lymphoproliferative disorders. The PI FA was positive in 5 of 18 diseased dogs with normal platelet counts. There was an inverse relationship between the platelet count and the intensity of fluorescence in the PIFA‐positive dogs. We conclude that the PIFA is a sensitive screening method for detecting circulating antiplatelet antibody.

Original languageEnglish (US)
Pages (from-to)36-39
Number of pages4
JournalJournal of veterinary internal medicine
Volume8
Issue number1
DOIs
StatePublished - Jan 1 1994

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antibody detection
fluorescent antibody technique
Fluorescent Antibody Technique
Blood Platelets
Dogs
Antibodies
dogs
assays
blood platelet count
thrombocytopenia
Platelet Count
Thrombocytopenia
Dog Diseases
megakaryocytes
Megakaryocytes
Lymphoproliferative Disorders
lupus erythematosus
lymphatic diseases
neoplasms
antibodies

Cite this

Detection of Antiplatelet Antibody With a Platelet Immunofluorescence Assay. / Kristensen, Annemarie T.; Weiss, Douglas J.; Klausner, Jeffrey S; Laber, Judy; Christie, Douglas J.

In: Journal of veterinary internal medicine, Vol. 8, No. 1, 01.01.1994, p. 36-39.

Research output: Contribution to journalArticle

Kristensen, Annemarie T. ; Weiss, Douglas J. ; Klausner, Jeffrey S ; Laber, Judy ; Christie, Douglas J. / Detection of Antiplatelet Antibody With a Platelet Immunofluorescence Assay. In: Journal of veterinary internal medicine. 1994 ; Vol. 8, No. 1. pp. 36-39.
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