Abstract
The nucleocapsid (N) protein of subgroup C (United States-specific) avian pneumovirus (APV/US) was expressed in Escherichia coli, and antibodies to the recombinant N protein were shown to specifically recognize the ≈47-kDa N protein of APV/US by Western immunoblot analysis. The recombinant APV/US N protein was used in a sandwich-capture enzyme-linked immunosorbent assay (ELISA), and the resulting assay was found to be more sensitive and specific than the routine indirect ELISA for the detection of APV/US antibodies in turkey sera.
Original language | English (US) |
---|---|
Pages (from-to) | 2967-2970 |
Number of pages | 4 |
Journal | Journal of clinical microbiology |
Volume | 39 |
Issue number | 8 |
DOIs | |
State | Published - 2001 |