Abstract
This unit provides procedures for detecting an quantifying radiolabeled proteins in SDS-polyacrylamide gels or blots. Autoradiography requires fixed, dried gels before exposure to X-ray film. The results may be enhanced by using intensifying screens and/or preflashing the film. Alternatively fluorography with organic scintillant-impregnated gels may be used to enhance signal. The autoradiogram may be quantified by densitometry. Phosphorimaging is a useful alternative to film exposure.
| Original language | English (US) |
|---|---|
| Journal | Current protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.] |
| Volume | Appendix 3 |
| DOIs | |
| State | Published - May 1 2001 |
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