Detection and partial genome sequence of a new umbra-like virus of papaya discovered in Ecuador

D. F. Quito-Avila; R. A. Alvarez; M. A. Ibarra; R. R. Martin

doi:10.1007/s10658-015-0675-y

Detection and partial genome sequence of a new umbra-like virus of papaya discovered in Ecuador

D. F. Quito-Avila, R. A. Alvarez, M. A. Ibarra, R. R. Martin

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Sequencing of cDNA synthesized from double-stranded RNA (dsRNA) from papaya leaves revealed the presence of a putative new virus with homology to genomes of several members of the genus Umbravirus. A wide survey showed that the virus was present in two out of three papaya producing provinces in Ecuador included in this study. The virus was found in up to 31 % of symptomatic trees co-infected with Papaya ringspot virus (PRSV) compared to 2 % in symptomless PRSV-free plants. Mechanical and aphid inoculations failed to transmit the virus under controlled conditions. The virus was not transmitted through seeds. The presence of a luteovirus, commonly associated with umbraviruses, was not confirmed in plants infected with the new virus using universal luteovirus primers.

Original language	English (US)
Pages (from-to)	199-204
Number of pages	6
Journal	European Journal of Plant Pathology
Volume	143
Issue number	1
DOIs	https://doi.org/10.1007/s10658-015-0675-y
State	Published - Sep 11 2015
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2015, Koninklijke Nederlandse Planteziektenkundige Vereniging.

Keywords

Ecuador
Papaya
Ringspot
Umbravirus

Publisher link

10.1007/s10658-015-0675-y

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title = "Detection and partial genome sequence of a new umbra-like virus of papaya discovered in Ecuador",

abstract = "Sequencing of cDNA synthesized from double-stranded RNA (dsRNA) from papaya leaves revealed the presence of a putative new virus with homology to genomes of several members of the genus Umbravirus. A wide survey showed that the virus was present in two out of three papaya producing provinces in Ecuador included in this study. The virus was found in up to 31 % of symptomatic trees co-infected with Papaya ringspot virus (PRSV) compared to 2 % in symptomless PRSV-free plants. Mechanical and aphid inoculations failed to transmit the virus under controlled conditions. The virus was not transmitted through seeds. The presence of a luteovirus, commonly associated with umbraviruses, was not confirmed in plants infected with the new virus using universal luteovirus primers.",

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note = "Publisher Copyright: {\textcopyright} 2015, Koninklijke Nederlandse Planteziektenkundige Vereniging.",

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doi = "10.1007/s10658-015-0675-y",

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T1 - Detection and partial genome sequence of a new umbra-like virus of papaya discovered in Ecuador

AU - Quito-Avila, D. F.

AU - Alvarez, R. A.

AU - Ibarra, M. A.

AU - Martin, R. R.

PY - 2015/9/11

Y1 - 2015/9/11

N2 - Sequencing of cDNA synthesized from double-stranded RNA (dsRNA) from papaya leaves revealed the presence of a putative new virus with homology to genomes of several members of the genus Umbravirus. A wide survey showed that the virus was present in two out of three papaya producing provinces in Ecuador included in this study. The virus was found in up to 31 % of symptomatic trees co-infected with Papaya ringspot virus (PRSV) compared to 2 % in symptomless PRSV-free plants. Mechanical and aphid inoculations failed to transmit the virus under controlled conditions. The virus was not transmitted through seeds. The presence of a luteovirus, commonly associated with umbraviruses, was not confirmed in plants infected with the new virus using universal luteovirus primers.

AB - Sequencing of cDNA synthesized from double-stranded RNA (dsRNA) from papaya leaves revealed the presence of a putative new virus with homology to genomes of several members of the genus Umbravirus. A wide survey showed that the virus was present in two out of three papaya producing provinces in Ecuador included in this study. The virus was found in up to 31 % of symptomatic trees co-infected with Papaya ringspot virus (PRSV) compared to 2 % in symptomless PRSV-free plants. Mechanical and aphid inoculations failed to transmit the virus under controlled conditions. The virus was not transmitted through seeds. The presence of a luteovirus, commonly associated with umbraviruses, was not confirmed in plants infected with the new virus using universal luteovirus primers.

KW - Ecuador

KW - Papaya

KW - Ringspot

KW - Umbravirus

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Detection and partial genome sequence of a new umbra-like virus of papaya discovered in Ecuador

Abstract

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