Detection and molecular characterization of kobuvirus from diarrheic goats in Minnesota

Nader M. Sobhy, Aníbal G. Armién, Arno Wünschmann, Dean Muldoon, Sagar M. Goyal, Sunil K. Mor

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Kobuvirus infections are common among humans, rodents, carnivores, pigs, and ruminants. We report herein the complete genome sequence of a novel caprine kobuvirus (MN604700) from diarrheic kids in Minnesota. Whole-genome sequencing revealed a kobuvirus genome of 8,139 nt with a single ORF region encoding a polyprotein of 2,480 amino acids. Further analysis revealed nt substitutions along the genome compared with that of the caprine kobuvirus reference strain, with 93% identity. Phylogenetic analysis indicated that the clade of the caprine kobuvirus was most closely related to porcine kobuviruses rather than bovine or ovine kobuviruses. Using primers designed from this genome, caprine kobuvirus was identified in the stools of other goats. Sanger sequencing of PCR products indicated 3D and VP1 gene nucleotides of this latter strain were 95% and 91% identical with those of MN604700, respectively. There were 35 and 101 nt substitutions in 3D and VP1 genes, respectively. Findings of kobuvirus over a 2-y period may indicate an endemic state, which needs further research. In addition, screening for kobuviruses over large geographic areas is needed to identify the evolutionary connections among different strains.

Original languageEnglish (US)
Pages (from-to)873-879
Number of pages7
JournalJournal of Veterinary Diagnostic Investigation
Issue number6
StatePublished - Nov 1 2020

Bibliographical note

Funding Information:
We thank Wendy Wiese and Lotus Smasal of the Virology Section of the Minnesota Veterinary Diagnostic Laboratory. The authors received no financial support for the research, authorship, and/or publication of this article.

Publisher Copyright:
© 2020 The Author(s).


  • diarrhea
  • electron microscopy
  • goats
  • kobuvirus
  • whole-genome sequence

PubMed: MeSH publication types

  • Journal Article


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