Abstract
A begomovirus and its associated betasatellites were amplified and sequenced from tobacco plants affected with leaf curl disease. The begomovirus was identified as a new strain of tomato leaf curl Pakistan virus (ToLCPKV), which is referred to here as ToLCPKV-India. A previously known betasatellite [tomato leaf curl Patna betasatellite (ToLCPaB)] and a new betasatellite were also found in leaf-curl-affected samples. The use of infectious clones of ToLCPKV-IN plus ToLCPaB for agroinoculation led to typical leaf curl, while ToLCPKV-IN together with the new betasatellite resulted in curling and chlorosis of leaves. Based on these disease symptoms, we propose to name the new betasatellite tobacco leaf chlorosis betasatellite (TbLChB).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 257-261 |
| Number of pages | 5 |
| Journal | Archives of Virology |
| Volume | 158 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2013 |
| Externally published | Yes |
Bibliographical note
Funding Information:Department of Biotechnology, Government of India, is acknowledged for financial support. Thanks to Birendra Singh (Skane University Hospital, Sweden) for critical reading of the manuscript.
Keywords
- Begomovirus
- Betasatellite
- Geminivirus
- Phylogenetic analysis
- Recombination