Detection and Assessment of MicroRNA Expression in Human Disease

Adam Mitchell, Amanda Marie James, Tamas Alexy, Gang Bao, Charles D. Searles

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

MicroRNAs (miRNAs) are noncoding RNAs that posttranscriptionally suppress gene expression through sequence-specific interaction with the 3′ untranslated region (3′UTR) of target mRNAs. By modulating gene networks, miRNAs have been shown to regulate many aspects of cellular homeostasis and physiology, including differentiation, growth, proliferation, and apoptosis. The discovery of extracellular miRNA in blood and other body fluids has prompted investigation into their ability to serve as biomarkers for human disease. Further, many miRNAs have been implicated in the underlying pathophysiology of human disease, and there is substantial opportunity to develop novel molecular therapeutics through manipulation of these miRNAs. Here, we review the evidence for the use of miRNAs as diagnostic and therapeutic targets in human disease. We also describe the advantages and limitations for current miRNA profiling strategies.

Original languageEnglish (US)
Title of host publicationRNA Technologies
PublisherSpringer Science and Business Media Deutschland GmbH
Pages333-349
Number of pages17
DOIs
StatePublished - 2015
Externally publishedYes

Publication series

NameRNA Technologies
ISSN (Print)2197-9731
ISSN (Electronic)2197-9758

Bibliographical note

Funding Information:
Acknowledgments This work was supported by a VA Merit Award (I01 BX000704 to CDS) and the National Heart Lung and Blood Institute of the National Institutes of Health as a Program of Excellence in Nanotechnology (HHSN268201000043C to GB) and a NHLBI R01 Award (HL 109559 to CDS).

Publisher Copyright:
© 2015, Springer International Publishing Switzerland.

Keywords

  • Biomarker
  • IsomiR
  • MicroRNA
  • Microarray
  • Molecular beacons
  • Next-generation sequencing
  • Northern
  • qRT-PCR

Fingerprint

Dive into the research topics of 'Detection and Assessment of MicroRNA Expression in Human Disease'. Together they form a unique fingerprint.

Cite this