Detailed method for intrathecal delivery of gene therapeutics by direct lumbar puncture in mice

Kelsey R. Pflepsen, Cristina D. Peterson, Kelley F Kitto, Lucy Vulchanova, George L Wilcox, Carolyn A Fairbanks

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Delivery of viral vectors directly into the central nervous system (CNS) has emerged as an important tool for the refinement of gene therapy. Intrathecal delivery by direct lumbar puncture in conscious rodents offers a minimally invasive approach that avoids tissue damage and/or destruction. Here we describe delivery of small quantities of viral vector product to the intrathecal space of rodents via direct lumbar puncture aided by a catheter.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages305-312
Number of pages8
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1937
ISSN (Print)1064-3745

Fingerprint

Spinal Puncture
Rodentia
Genetic Therapy
Genes
Catheters
Central Nervous System
Therapeutics

Keywords

  • AAV gene therapy
  • Direct lumbar puncture
  • Gene therapy
  • Intrathecal
  • Intrathecal gene delivery

Cite this

Pflepsen, K. R., Peterson, C. D., Kitto, K. F., Vulchanova, L., Wilcox, G. L., & Fairbanks, C. A. (2019). Detailed method for intrathecal delivery of gene therapeutics by direct lumbar puncture in mice. In Methods in Molecular Biology (pp. 305-312). (Methods in Molecular Biology; Vol. 1937). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9065-8_20

Detailed method for intrathecal delivery of gene therapeutics by direct lumbar puncture in mice. / Pflepsen, Kelsey R.; Peterson, Cristina D.; Kitto, Kelley F; Vulchanova, Lucy; Wilcox, George L; Fairbanks, Carolyn A.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 305-312 (Methods in Molecular Biology; Vol. 1937).

Research output: Chapter in Book/Report/Conference proceedingChapter

Pflepsen, KR, Peterson, CD, Kitto, KF, Vulchanova, L, Wilcox, GL & Fairbanks, CA 2019, Detailed method for intrathecal delivery of gene therapeutics by direct lumbar puncture in mice. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1937, Humana Press Inc., pp. 305-312. https://doi.org/10.1007/978-1-4939-9065-8_20
Pflepsen KR, Peterson CD, Kitto KF, Vulchanova L, Wilcox GL, Fairbanks CA. Detailed method for intrathecal delivery of gene therapeutics by direct lumbar puncture in mice. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 305-312. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9065-8_20
Pflepsen, Kelsey R. ; Peterson, Cristina D. ; Kitto, Kelley F ; Vulchanova, Lucy ; Wilcox, George L ; Fairbanks, Carolyn A. / Detailed method for intrathecal delivery of gene therapeutics by direct lumbar puncture in mice. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 305-312 (Methods in Molecular Biology).
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