Delivery of viral vectors directly into the central nervous system (CNS) has emerged as an important tool for the refinement of gene therapy. Intrathecal delivery by direct lumbar puncture in conscious rodents offers a minimally invasive approach that avoids tissue damage and/or destruction. Here we describe delivery of small quantities of viral vector product to the intrathecal space of rodents via direct lumbar puncture aided by a catheter.
|Original language||English (US)|
|Title of host publication||Methods in Molecular Biology|
|Publisher||Humana Press Inc.|
|Number of pages||8|
|State||Published - 2019|
|Name||Methods in molecular biology (Clifton, N.J.)|
Bibliographical noteFunding Information:
This work was supported by NIDA grant R01DA035931-01, DoD grant W81XWH-15-1-0494, T32 training grant T32DA07234 to K.P., and T32 training grant T32DA007097 to C.P.
- AAV gene therapy
- Direct lumbar puncture
- Gene therapy
- Intrathecal gene delivery
- Injections, Spinal
- Genetic Therapy
- Genetic Vectors/administration & dosage
- Spinal Puncture/instrumentation
PubMed: MeSH publication types
- Research Support, U.S. Gov't, Non-P.H.S.
- Journal Article
- Research Support, N.I.H., Extramural