Detailed deletion mapping of chromosome 9p21-22 in nasopharyngeal carcinoma

Jian Bo Yang; Xiao Mei Zhang; Long Wen Deng; Guo Lin Tan; Ming Zhou; Zhao Yang Zeng; Li Cao; Shou Rong Shen; Gui Yuan Li

doi:10.1007/BF02983458

Detailed deletion mapping of chromosome 9p21-22 in nasopharyngeal carcinoma

Jian Bo Yang, Xiao Mei Zhang, Long Wen Deng, Guo Lin Tan, Ming Zhou, Zhao Yang Zeng, Li Cao, Shou Rong Shen, Gui Yuan Li

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Objective: To further refine the extent of deletion on chromosome 9p21-22 in nasopharyngeal carcinoma (NPC) and provide evidence for discovering new tumor suppressor gene. Methods: Loss of heterozygosity (LOH) on chromosome 9p21-22 was analyzed in 25 paired blood and tumor samples by using 11 high-density microsatellite polymorphic markers. Results: 17 of 25 cases (68.0%) showed LOH at one or more loci. Higher frequencies of LOH were found at four loci: D9S161 (35.0%), D9S1678 (31.5%), D9S263 (33.3%) and D9S1853 (33.3%), where 6 cases had a contiguous stretch of allelic loss. Conclusion: The minimal common region of deletion might be defined between D9S161 and D9S1853 (estimated about 2.7 cM in extent) at 9p21.1, suggesting that inactivation of one or more tumor suppressor genes located in this region may be an important step in NPC.

Original language	English (US)
Pages (from-to)	161-164
Number of pages	4
Journal	Chinese Journal of Cancer Research
Volume	12
Issue number	3
DOIs	https://doi.org/10.1007/BF02983458
State	Published - 2000

Bibliographical note

Funding Information:
Previous studies have showed that Epstein-Barr virus (EBV) infection, certain environmental factors and genetic factors were found to be closely associated with nasopharyngeal carcinoma. The rates of NPC in southern China and Southeast Asia are 25 times higher than that of Received April 10, 2000, accepted July 17, 2000 This work was supported by a grant from the National "863" Project of China (No. 102-10-01-05). Correspondence to: LI Gui-yuan, Cancer Research Institute, Hunan Medical University, No. 88, Xiangya Road, Changsha 410078, Hunan, China; Phone: (0086-731)-4805446; Fax: (0086-731)-4805383; E-mail: ligy@pubfic.cs.hn.cn in western countries. The statistic analysis revealed 5%-10% NPC patients have family history, there, genetic susceptibility might be an important factor in the pathogenesis of NPC. Unfortunately the alterations of common tumor suppressor genes are rare in NPC. Previous cytogenetic and molecular genetic studies have showed that loss of heterozygosity (LOH) on chromosome 9p21-22 is one of the most frequent genetic alterations in NPC and in many common sporadic cancers which suggested that there may be more than one TSG on 9p21-22. The pl6/MTS1 gene located at 9p21.has been considered a putative tumor suppressor gene at this region. Although homozygous deletion and point mutations of p l6/MTS1 occur frequently in a variety of human tumor cell lines, corresponding evidence of the same alternations in primary NPC samples is less common. To further precisely the refine extent of deletion on chromosome 9p21-22 in NPC, in this study, we performed loss of heterozygosity analysis on chromosome 9p21-22 in 25 paired blood and tumor samples using 11 high-density microsatellite polymorphic markers.

Keywords

Chromosome 9p21-22
Loss of heterozygosity
Nasopharyngeal carcinoma
Tumor suppressor gene

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@article{d12d1acf6e3447cea6b97bfe3067bd68,

title = "Detailed deletion mapping of chromosome 9p21-22 in nasopharyngeal carcinoma",

abstract = "Objective: To further refine the extent of deletion on chromosome 9p21-22 in nasopharyngeal carcinoma (NPC) and provide evidence for discovering new tumor suppressor gene. Methods: Loss of heterozygosity (LOH) on chromosome 9p21-22 was analyzed in 25 paired blood and tumor samples by using 11 high-density microsatellite polymorphic markers. Results: 17 of 25 cases (68.0%) showed LOH at one or more loci. Higher frequencies of LOH were found at four loci: D9S161 (35.0%), D9S1678 (31.5%), D9S263 (33.3%) and D9S1853 (33.3%), where 6 cases had a contiguous stretch of allelic loss. Conclusion: The minimal common region of deletion might be defined between D9S161 and D9S1853 (estimated about 2.7 cM in extent) at 9p21.1, suggesting that inactivation of one or more tumor suppressor genes located in this region may be an important step in NPC.",

keywords = "Chromosome 9p21-22, Loss of heterozygosity, Nasopharyngeal carcinoma, Tumor suppressor gene",

author = "Yang, {Jian Bo} and Zhang, {Xiao Mei} and Deng, {Long Wen} and Tan, {Guo Lin} and Ming Zhou and Zeng, {Zhao Yang} and Li Cao and Shen, {Shou Rong} and Li, {Gui Yuan}",

note = "Funding Information: Previous studies have showed that Epstein-Barr virus (EBV) infection, certain environmental factors and genetic factors were found to be closely associated with nasopharyngeal carcinoma. The rates of NPC in southern China and Southeast Asia are 25 times higher than that of Received April 10, 2000, accepted July 17, 2000 This work was supported by a grant from the National {"}863{"} Project of China (No. 102-10-01-05). Correspondence to: LI Gui-yuan, Cancer Research Institute, Hunan Medical University, No. 88, Xiangya Road, Changsha 410078, Hunan, China; Phone: (0086-731)-4805446; Fax: (0086-731)-4805383; E-mail: ligy@pubfic.cs.hn.cn in western countries. The statistic analysis revealed 5%-10% NPC patients have family history, there, genetic susceptibility might be an important factor in the pathogenesis of NPC. Unfortunately the alterations of common tumor suppressor genes are rare in NPC. Previous cytogenetic and molecular genetic studies have showed that loss of heterozygosity (LOH) on chromosome 9p21-22 is one of the most frequent genetic alterations in NPC and in many common sporadic cancers which suggested that there may be more than one TSG on 9p21-22. The pl6/MTS1 gene located at 9p21.has been considered a putative tumor suppressor gene at this region. Although homozygous deletion and point mutations of p l6/MTS1 occur frequently in a variety of human tumor cell lines, corresponding evidence of the same alternations in primary NPC samples is less common. To further precisely the refine extent of deletion on chromosome 9p21-22 in NPC, in this study, we performed loss of heterozygosity analysis on chromosome 9p21-22 in 25 paired blood and tumor samples using 11 high-density microsatellite polymorphic markers.",

year = "2000",

doi = "10.1007/BF02983458",

language = "English (US)",

volume = "12",

pages = "161--164",

journal = "Chinese Journal of Cancer Research",

issn = "1000-9604",

publisher = "Beijing Institute for Cancer Research",

number = "3",

}

TY - JOUR

T1 - Detailed deletion mapping of chromosome 9p21-22 in nasopharyngeal carcinoma

AU - Yang, Jian Bo

AU - Zhang, Xiao Mei

AU - Deng, Long Wen

AU - Tan, Guo Lin

AU - Zhou, Ming

AU - Zeng, Zhao Yang

AU - Cao, Li

AU - Shen, Shou Rong

AU - Li, Gui Yuan

N1 - Funding Information: Previous studies have showed that Epstein-Barr virus (EBV) infection, certain environmental factors and genetic factors were found to be closely associated with nasopharyngeal carcinoma. The rates of NPC in southern China and Southeast Asia are 25 times higher than that of Received April 10, 2000, accepted July 17, 2000 This work was supported by a grant from the National "863" Project of China (No. 102-10-01-05). Correspondence to: LI Gui-yuan, Cancer Research Institute, Hunan Medical University, No. 88, Xiangya Road, Changsha 410078, Hunan, China; Phone: (0086-731)-4805446; Fax: (0086-731)-4805383; E-mail: ligy@pubfic.cs.hn.cn in western countries. The statistic analysis revealed 5%-10% NPC patients have family history, there, genetic susceptibility might be an important factor in the pathogenesis of NPC. Unfortunately the alterations of common tumor suppressor genes are rare in NPC. Previous cytogenetic and molecular genetic studies have showed that loss of heterozygosity (LOH) on chromosome 9p21-22 is one of the most frequent genetic alterations in NPC and in many common sporadic cancers which suggested that there may be more than one TSG on 9p21-22. The pl6/MTS1 gene located at 9p21.has been considered a putative tumor suppressor gene at this region. Although homozygous deletion and point mutations of p l6/MTS1 occur frequently in a variety of human tumor cell lines, corresponding evidence of the same alternations in primary NPC samples is less common. To further precisely the refine extent of deletion on chromosome 9p21-22 in NPC, in this study, we performed loss of heterozygosity analysis on chromosome 9p21-22 in 25 paired blood and tumor samples using 11 high-density microsatellite polymorphic markers.

PY - 2000

Y1 - 2000

N2 - Objective: To further refine the extent of deletion on chromosome 9p21-22 in nasopharyngeal carcinoma (NPC) and provide evidence for discovering new tumor suppressor gene. Methods: Loss of heterozygosity (LOH) on chromosome 9p21-22 was analyzed in 25 paired blood and tumor samples by using 11 high-density microsatellite polymorphic markers. Results: 17 of 25 cases (68.0%) showed LOH at one or more loci. Higher frequencies of LOH were found at four loci: D9S161 (35.0%), D9S1678 (31.5%), D9S263 (33.3%) and D9S1853 (33.3%), where 6 cases had a contiguous stretch of allelic loss. Conclusion: The minimal common region of deletion might be defined between D9S161 and D9S1853 (estimated about 2.7 cM in extent) at 9p21.1, suggesting that inactivation of one or more tumor suppressor genes located in this region may be an important step in NPC.

AB - Objective: To further refine the extent of deletion on chromosome 9p21-22 in nasopharyngeal carcinoma (NPC) and provide evidence for discovering new tumor suppressor gene. Methods: Loss of heterozygosity (LOH) on chromosome 9p21-22 was analyzed in 25 paired blood and tumor samples by using 11 high-density microsatellite polymorphic markers. Results: 17 of 25 cases (68.0%) showed LOH at one or more loci. Higher frequencies of LOH were found at four loci: D9S161 (35.0%), D9S1678 (31.5%), D9S263 (33.3%) and D9S1853 (33.3%), where 6 cases had a contiguous stretch of allelic loss. Conclusion: The minimal common region of deletion might be defined between D9S161 and D9S1853 (estimated about 2.7 cM in extent) at 9p21.1, suggesting that inactivation of one or more tumor suppressor genes located in this region may be an important step in NPC.

KW - Chromosome 9p21-22

KW - Loss of heterozygosity

KW - Nasopharyngeal carcinoma

KW - Tumor suppressor gene

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Detailed deletion mapping of chromosome 9p21-22 in nasopharyngeal carcinoma

Abstract

Bibliographical note

Keywords

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