Abstract
Advances in DNA synthesis and assembly technology allow for the high-throughput fabrication of hundreds to thousands of multi-part genetic constructs in a short time. This allows for rapid hypothesis-testing and genetic optimization in multi-gene biological systems. Here, we discuss key considerations to design and implement an algorithmic DNA assembly pipeline that provides the freedom to change nearly any design variable in a multi-gene system. In addition to considerations for pipeline design, we describe protocols for three useful molecular biology techniques in plasmid construction.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 131-147 |
| Number of pages | 17 |
| DOIs | |
| State | Published - Jan 1 2018 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1671 |
| ISSN (Print) | 1064-3745 |
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Keywords
- BioDesign automation
- DNA assembly
- Genetic refactoring
- Golden Gate assembly
- Isothermal assembly
- PCR-ligation
PubMed: MeSH publication types
- Journal Article
- Research Support, Non-U.S. Gov't
Cite this
Designing and implementing algorithmic DNA assembly pipelines for multi-gene systems. / Hsu, Szu Yi; Smanski, Michael J.
Methods in Molecular Biology. Humana Press Inc., 2018. p. 131-147 (Methods in Molecular Biology; Vol. 1671).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Designing and implementing algorithmic DNA assembly pipelines for multi-gene systems
AU - Hsu, Szu Yi
AU - Smanski, Michael J
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Advances in DNA synthesis and assembly technology allow for the high-throughput fabrication of hundreds to thousands of multi-part genetic constructs in a short time. This allows for rapid hypothesis-testing and genetic optimization in multi-gene biological systems. Here, we discuss key considerations to design and implement an algorithmic DNA assembly pipeline that provides the freedom to change nearly any design variable in a multi-gene system. In addition to considerations for pipeline design, we describe protocols for three useful molecular biology techniques in plasmid construction.
AB - Advances in DNA synthesis and assembly technology allow for the high-throughput fabrication of hundreds to thousands of multi-part genetic constructs in a short time. This allows for rapid hypothesis-testing and genetic optimization in multi-gene biological systems. Here, we discuss key considerations to design and implement an algorithmic DNA assembly pipeline that provides the freedom to change nearly any design variable in a multi-gene system. In addition to considerations for pipeline design, we describe protocols for three useful molecular biology techniques in plasmid construction.
KW - BioDesign automation
KW - DNA assembly
KW - Genetic refactoring
KW - Golden Gate assembly
KW - Isothermal assembly
KW - PCR-ligation
UR - http://www.scopus.com/inward/record.url?scp=85035135090&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85035135090&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-7295-1_9
DO - 10.1007/978-1-4939-7295-1_9
M3 - Chapter
T3 - Methods in Molecular Biology
SP - 131
EP - 147
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -