@inbook{c276c58c068a4866b3e5034a9dd664c6,
title = "Designing and implementing algorithmic DNA assembly pipelines for multi-gene systems",
abstract = "Advances in DNA synthesis and assembly technology allow for the high-throughput fabrication of hundreds to thousands of multi-part genetic constructs in a short time. This allows for rapid hypothesis-testing and genetic optimization in multi-gene biological systems. Here, we discuss key considerations to design and implement an algorithmic DNA assembly pipeline that provides the freedom to change nearly any design variable in a multi-gene system. In addition to considerations for pipeline design, we describe protocols for three useful molecular biology techniques in plasmid construction.",
keywords = "BioDesign automation, DNA assembly, Genetic refactoring, Golden Gate assembly, Isothermal assembly, PCR-ligation",
author = "Hsu, {Szu Yi} and Smanski, {Michael J.}",
year = "2018",
month = jan,
day = "1",
doi = "10.1007/978-1-4939-7295-1_9",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "131--147",
booktitle = "Methods in Molecular Biology",
}