Designed β-sheet peptides that inhibit proliferation and induce apoptosis in endothelial cells

Kevin H. Mayo, Daisy W.J. Van Der Schaft, Arjan W. Griffioen

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Novel β-sheet-forming peptide 33mers, βpep peptides, have been designed by using a combination approach employing basic folding principles and incorporating short sequences or proposed key residues from the β-sheet domains of interleukin-8 (IL-8), platelet factor-4 (PF4) and bactericidal/permeability increasing protein (B/PI), Since PF4 and B/PI are anti-angiogenic and IL-8 is angiogenic, the library of 30 βpep peptides was assayed for the ability to affect the growth of endothelial cells, Results indicate that five βpep peptides (βpep-2, 7, 8, 21 and 25) demonstrate greater than 50% anti-proliferative activity at 30 μg/ml, and one of those (βpep-25) is similarly active at 10 μg/ml, Insight into the mechanism of action was probed in an apoptosis assay. Anti-proliferative activity was found to be correlated with the induction of apoptosis, For example, at 100 μg/ml βpep-25 induces 85% of endothelial cells to undergo apoptosis within 2 days, These effects from βpep peptides appear to be selective for endothelial cell (EC) because normal cells (fibroblasts and leukocytes) and various tumor cells are not significantly affected at peptide concentrations used in this study, Comparison of active and inactive βpep sequences allows structure-function relationships to be deduced. Five hydrophobic residues and two lysines appear to be crucial to activity. This research contributes to the development of novel anti-angiogenic peptides.

Original languageEnglish (US)
Pages (from-to)45-51
Number of pages7
JournalAngiogenesis
Volume4
Issue number1
DOIs
StatePublished - 2001

Bibliographical note

Funding Information:
This work was supported by generous research grants from Department of Defense (Army) DAMD17-99-1-9564, ActiPep Biotechnology, Inc., and the Profiler-ingsfonds azM of the University Hospital Maastricht. We are grateful to Denisha Walik of the Microchemical Facility for expertise in the synthesis of peptides.

Keywords

  • Activity
  • Angiogenesis
  • Peptide
  • Structure

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