Structural studies of topoisomerase-fluoroquinolone-DNA ternary complexes revealed a cavity between the quinolone N-1 position and the active site tyrosine. Fluoroquinolone derivatives having positively charged or aromatic moieties extended from the N-1 position were designed to probe for binding contacts with the phosphotyrosine residue in ternary complex. While alkylamine, alkylphthalimide, and alkylphenyl groups introduced at the N-1 position afforded derivatives that maintained modest inhibition of the supercoiling activity of DNA gyrase, none retained ability to poison DNA gyrase. Thus, the addition of a large and/or long moiety at the N-1 position disrupts ternary complex formation, and retained ability to inhibit supercoiling is likely through interference with the strand breakage reaction. Two derivatives were found to possess inhibitory effects on the decatenation activity of human topoisomerase II.
|Original language||English (US)|
|Number of pages||8|
|Journal||Bioorganic and Medicinal Chemistry Letters|
|State||Published - Jun 1 2018|
Bibliographical noteFunding Information:
This work was supported by National Institutes of Health (NIH) Research Grant R01 AI87671 to RJK. Tyrell Towle acknowledges support of the NIH Predoctoral Training Program in Pharmacological Sciences (GM067795), the American Foundation for Pharmaceutical Education Predoctoral Fellowship Program and the American Chemical Society Division of Medicinal Chemistry Fellowship sponsored by Richard B. Silverman—2012 Predoctoral. Chaitanya Kulkarni acknowledges the support of a training fellowship from the University of Iowa Center for Biocatalysis and Bioprocessing and of the NIH-sponsored Predoctoral Training Program in Biotechnology (GM008365).
© 2018 Elsevier Ltd
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